Mouse ADAM17 / TACE / CD156b Protein, His Tag

分子別名（Synonym）

ADAM17,TACE,CD156b

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse ADAM17, His Tag (AD7-M52H1) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Asp 563 (Accession # Q9Z0F8).

Predicted N-terminus: Pro 18, Asp 59 and Arg 215

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 63.4 kDa. The protein migrates as 19 kDa and 20 kDa (Propeptide), 55-66 kDa (Mature-ADAM17) and 75-94 kDa (Pro-ADAM17) under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM MES, 150 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

含去整合素和金屬蛋白酶結(jié)構(gòu)域的蛋白17（ADAM17）是去整合素和金蛋白酶ADAM蛋白家族的成員，也稱為TNF-α轉(zhuǎn)化酶、TNF-α轉(zhuǎn)換酶和CD156b，CD156b含有一個(gè)去整合素結(jié)構(gòu)域和一個(gè)肽酶M12B結(jié)構(gòu)域。ADAM17可以將TNFα的膜結(jié)合前體切割成其成熟的可溶性形式。ADAM17還負(fù)責(zé)可溶性JAM3從內(nèi)皮細(xì)胞表面的蛋白水解釋放（通過相似性）和其他幾種細(xì)胞表面蛋白的蛋白水解分泌，包括p75 TNF受體、白細(xì)胞介素1受體II型、p55 TNF受體、轉(zhuǎn)化生長(zhǎng)因子α、L-選擇素、生長(zhǎng)激素受體、MUC1和淀粉樣前體蛋白。此外，ADAM17通過介導(dǎo)Notch的切割，產(chǎn)生稱為Notch細(xì)胞外截短的膜相關(guān)中間片段，從而充當(dāng)Notch通路的激活劑。