Mouse IL-10 Protein, His Tag

分子別名（Synonym）

CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse IL-10, His Tag (IL0-M4248) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Ser 178 (Accession # NP_034678).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.6 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>92% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素-10（IL-10）也稱為人類細(xì)胞因子合成抑制因子（CSIF），是一種抗炎細(xì)胞因子。IL-10是一種免疫抑制細(xì)胞因子，由多種哺乳動物細(xì)胞類型產(chǎn)生，包括巨噬細(xì)胞、單核細(xì)胞、T細(xì)胞、B細(xì)胞和角質(zhì)形成細(xì)胞。成熟的人IL-10與牛、犬、馬、貓、小鼠、綿羊、豬和大鼠IL-10具有72%-86%的氨基酸序列同一性。雖然人IL-10對小鼠細(xì)胞有活性，但小鼠IL-10不作用于人細(xì)胞。IL-10能夠抑制巨噬細(xì)胞和調(diào)節(jié)性T細(xì)胞等細(xì)胞產(chǎn)生的促炎細(xì)胞因子如IFN-γ、IL-2、IL-3、TNFα和GM-CSF的合成。它還顯示出抑制抗原呈遞細(xì)胞抗原呈遞能力的強(qiáng)大能力。然而，它對某些T細(xì)胞和肥大細(xì)胞也有刺激作用，并刺激B細(xì)胞成熟和抗體產(chǎn)生。敲除研究表明，白細(xì)胞介素-10/IL-10在腸道中起著重要的免疫調(diào)節(jié)作用?？肆_恩病患者對使用產(chǎn)生重組白細(xì)胞介素-10的細(xì)菌進(jìn)行治療反應(yīng)良好，這表明白細(xì)胞介蛋白-10對抵消人體過度免疫的重要性。