Unconjugated Human TPBG / 5T4 Protein, His,Avitag?

分子別名（Synonym）

TPBG,5T4,M6P1,5T4AG,WAIF1,5T4 oncofetal antigen,Trophoblast glycoprotein,5T4 oncofetal trophoblast glycoprotein

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Unconjugated Human TPBG, His,Avitag (TPG-H52E5) is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Ser 355 (Accession # Q13641-1).

Predicted N-terminus: Ser 32

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

應(yīng)用說明（Application）

TPG-H52E5 is the unconjugated version of TPG-H82Eb (Biotinylated Human TPBG, His,Avitag). It can be utilized for assay development or customized biotinylation according to the experimental designs.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

滋養(yǎng)層糖蛋白（TPBG），也稱為5T4，是目前臨床開發(fā)的幾種抗癌藥物的治療靶點(diǎn)，主要是由于其在腫瘤中的高表達(dá)和在正常成人組織中的低表達(dá)。該基因編碼一種富含亮氨酸的跨膜糖蛋白，可能參與細(xì)胞粘附。TPBG早在發(fā)育9周時(shí)就由所有類型的滋養(yǎng)層細(xì)胞表達(dá)。