Human IL-2 R gamma / CD132 Protein, His Tag (MALS verified)

分子別名（Synonym）

IL2RG,CD132,CIDX,IMD4,P64,SCIDX,SCIDX1,gammaC

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-2 R gamma, His Tag (ILA-H52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Asn 254 (Accession # P31785-1).

Predicted N-terminus: Leu 23

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 29.3 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

IL-2R是一種異三聚體蛋白，與細(xì)胞因子IL-2結(jié)合并產(chǎn)生反應(yīng)。鑒定出三條不同的IL-2R鏈，稱為α、β和γ，它們是非共價(jià)結(jié)合的。α鏈和β鏈參與結(jié)合IL-2，而細(xì)胞因子相互作用后的信號(hào)轉(zhuǎn)導(dǎo)由γ鏈和β亞基進(jìn)行。IL-2R的α鏈可以在受體與IL-2相互作用之前與β鏈結(jié)合。單獨(dú)的γ鏈對(duì)IL-2的親和力非常弱，但在配體與α/β異二聚體結(jié)合后，γ鏈被招募到復(fù)合物中，形成非常穩(wěn)定的大分子四元配體/受體復(fù)合物。

白細(xì)胞介素-2受體亞基γ（IL2RG），也稱為細(xì)胞因子受體共同亞基γ，CD抗原CD132，gammaC，p64，屬于I型細(xì)胞因子受體家族或5型亞家族。IL2RG位于骨髓中未成熟造血細(xì)胞的表面。IL2RG缺陷是嚴(yán)重聯(lián)合免疫缺陷X連鎖T細(xì)胞陰性/B細(xì)胞陽(yáng)性/NK細(xì)胞陰性（XSCID）的原因。