Human ICAM-1 / CD54 Protein, Fc Tag

分子別名（Synonym）

ICAM1,BB2,CD54,P3.58

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human ICAM-1, Fc Tag (IC1-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Glu 480 (Accession # AAH15969).

Predicted N-terminus: Gln 28

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 75.7 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

細(xì)胞間粘附分子1（ICAM-1）也稱為分化簇54（CD54），是免疫球蛋白超家族的成員，是一種細(xì)胞表面糖蛋白，通常在內(nèi)皮細(xì)胞和免疫系統(tǒng)細(xì)胞上以低濃度表達(dá)。該基因編碼的蛋白質(zhì)是一種細(xì)胞間粘附分子，以低濃度持續(xù)存在于白細(xì)胞和內(nèi)皮細(xì)胞的膜中。在細(xì)胞因子刺激下，濃度大大增加。ICAM-1可由白細(xì)胞介素-1（IL-1）和腫瘤壞死因子α（TNFα）誘導(dǎo)，并由血管內(nèi)皮、巨噬細(xì)胞和淋巴細(xì)胞表達(dá)。ICAM-1是白細(xì)胞上發(fā)現(xiàn)的受體LFA-1（整合素）的配體。當(dāng)被激活時(shí)，白細(xì)胞通過ICAM-1/LFA-1與內(nèi)皮細(xì)胞結(jié)合，然后轉(zhuǎn)移到組織中。ICAM-1與蛛網(wǎng)膜下腔出血（SAH）有關(guān)。許多研究表明，蛛網(wǎng)膜下腔出血患者的ICAM-1水平明顯高于對(duì)照組。呼吸道上皮細(xì)胞表達(dá)的ICAM-1也是鼻病毒的結(jié)合位點(diǎn)，鼻病毒是最常見感冒的病原體。