中文名：1,4-二硫蘇糖醇
英文名：1,4-Dithiothreitol (DTT)
中文別名：1,4-二巰基蘇糖醇, 克萊蘭氏試劑|DL-1,4-二硫蘇糖醇|二硫代蘇糖醇|DL-二硫蘇糖醇
英文別名：Dithiothreitol|DL-1,4-Dithiothreitol|dl-Dithiothreitol|3483-12-3|(2S,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL|1,4-Dithiothreitol|D-1,4-Dithiothreitol|D-Dithiothreitol|27565-41-9|D-Dtt|1,4-Dithio-dl-threitol|threo-1,4-Dimercapto-2,3-butanediol|(2S,3S)-1,4-disulfa
純度：≥97%
貨號(hào)：D265376
包裝：100g、1g、25g
Cas號(hào)：3483-12-3
存儲(chǔ)溫度：2-8°C儲(chǔ)存,避光,充氬
產(chǎn)品介紹：
Overview
Reducing agent
Application
Use 1,4-Dithiothreitol primarily to protect free SH-groups from oxidation. Use it routinely in all work with enzymes and proteins during enzyme measurement and the characterization of proteins.
? As one of the reduction and alkylation reactants of the main allergen αs1-casein in milk.
? As a component of sperm membrane removal and reactivation medium.
?Because mercaptans can effectively protect the active sites of biocatalysts and maintain the stability of enzymes.
? As a reducing agent to test the substitution of N-alkaline maleic substitution and the reaction of excess radicals.
Before SDS-PAGE, DTT can effectively reduce protein disulfide bonds in the sample buffer. Both the irritation and toxicity of DTT are reduced by 2-mercaptoethanol. Usually, the required concentration of DTT (100mM) is used. It uses one-seventh of the concentration of 2-cholesterol-based ethanol (5% v/v, 700 mM).
Benefits
1.Rely on the higher stability of DTT in comparison to 2-mercaptoethanol
in aqueous solution
2.Experience more convenience and a less disagreeable odor
3.Benefit from the reduced tendency to oxidize in air.
Specification
Appearance: White to yellowish crystallizate
DTT (with Ellman's reagent): ≥97%
Thin layer chromatography: Chromatographically homogeneous, corresponds to reference
Stability: At +2 to +8°C within specification range for 24 months. Store dry. Protect from light.
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關(guān)鍵字： 1,4-二巰基蘇糖醇, 克萊蘭氏試劑;DL-1,4-二硫蘇糖醇;二硫代蘇糖醇;DL-二硫蘇糖醇
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