中文名：畢卡魯胺
英文名：Bicalutamide (ICI-176334)
英文別名：N-?[4-?cyano-?3-?(trifluoromethyl)?phenyl]?-?3-?[(4-?fluorophenyl)?sulfonyl]?-?2-?hydroxy-?2-?methyl-propanamide
純度：10mM in DMSO
貨號：B407813
包裝：1ml
Cas號：90357-06-5
存儲溫度：-80℃儲存
產(chǎn)品介紹：
Information
Bicalutamide (ICI-176334) Bicalutamide (ICI-176334) is an androgen receptor (AR) antagonist with IC50 of 0.16 μM in LNCaP/AR(cs)cell line. Bicalutamide promotes autophagy .
In?vitro
Bicalutamide undergoes an antagonist-to-agonist switch, stimulating AR activity. Bicalutamide treatment of LNCaP/AR(cs) cells in absence of the synthetic androgen R1881 results in altered gene expression consistent with its well-documented agonist activity in context of AR overexpression. Bicalutamide induces cell proliferation in a dose-dependent manner, and only partially antagonized the effects of R1881. Bicalutamide treatment also results in a significant amount of nuclear AR, although less than that observed with R1881. Bicalutamide exhibits partial agonist activity as evidenced by induction of DNA binding at AR target genes and incomplete antagonism of the effects of R1881. In absence of R1881, Bicalutamide partially activates VP16-AR–mediated transcription, indicative of AR binding to DNA. In LNCaP/AR-luc cells with a stably integrates AR-driven luciferase reporter construct. In the presence of R1881, Bicalutamide shows only weak partial antagonism of VP16-AR–mediated transcription with an IC50 of 0.35 μM. Micromolar bicalutamide causes a significant dose-dependent reduction in clonogenicity. Dual inhibition of the AR and mTOR signaling pathways provides further benefit with the ridaforolimus-bicalutamide combination producing syner -gistic antiproliferative effects in prostate cancer cells in vitro when compared with each agent alone.
In?vivo
Single bicalutamide reduces tumor growth by 79%, at defined submaximal doses. The ridaforolimus-bicalutamide combination exhibits improved and potent antitumor activity, almost completely abrogating tumor growth. The combination is also well tolerated, as evidenced by no significant changes in body weight over the course of treatment. Plasma PSA levels are again tightly linked to tumor growth in the combination-treated mice.
Cell?Data
cell?lines：
Concentrations：0-1 μM
Incubation?Time：72 hours
Powder?Purity:≥99%
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關(guān)鍵字： N-?[4-?cyano-?3-?(trifluoromethyl)?phenyl]?-?3-?[(4-?fluorophenyl)?sulfonyl]?-?2-?hydroxy-?2-?methyl-propanamide
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