D-氨基葡萄糖,最新報(bào)價(jià):630.54元/MG,產(chǎn)品編號(hào)n9376品牌Sigma雜質(zhì)≤0.05%Freep-nitrophenol測(cè)定≥99%(TLC)形式powder底物N-乙酰-氨基葡糖苷酶的發(fā)色底物。別" /> D-氨基葡萄糖,3459-18-5,4-硝基苯基 N-乙?;?β-D-氨基葡萄糖價(jià)格,單糖" />
溶解性
water: 5?mg/mL, clear, colorless to very faintly yellow
應(yīng)用
N-acetyl-β-D-glucosaminidase (NAG) has been used:as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)as a substrate to study chitinase ChiB1 activityto assess degranulation using rat basophilic leukemia (RBL)-2H3 cells
生化/生理作用
4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.
Sigma-Aldrich西格瑪奧德里奇(上海)貿(mào)易有限公司
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