Description
DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.
Catalog No.
RGK60001
Host species
mouse
Tested applications
CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IP, SPR, IF
Species reactivity
General
Form
liquid
Storage buffer
0.01M PBS, pH 7.4.
Concentration
0.74 mg/ml
Purity
>95%
Clonality
Monoclonal
Isotype
IgG2a, kappa
Applications
CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IP, SPR, IF
Purification
Protein A or G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Expression system
Mammalian cells
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.
Reconstitution
Reconstitute in sterile water for a stock solution.
Alternative Names
DNA-RNA Hybrid
Note
For research use only.
Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids. PMID: 33830170
Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody. PMID: 32681514
High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing. PMID: 31053798
RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage. PMID: 29742442
The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences. PMID: 28594954
Structural basis of R-loop recognition by the S9.6 monoclonal antibody. PMID: 35347133
Best practices for the visualization, mapping, and manipulation of R-loops. PMID: 33411340
Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. PMID: 34232287
The Affinity of the S9.6 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast. PMID: 29289567
The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S9.6. PMID: 23784994
Modifications and interactions at the R-loop. PMID: 32961406
BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. PMID: 24896180
R-Loop Analysis by Dot-Blot. PMID: 33554969
Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead. PMID: 34324345
New insight into the biology of R-loops. PMID: 32516653
Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR. PMID: 33371452
Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation. PMID: 32105733
Complexities due to single-stranded RNA during antibody detection of genomic rna:dna hybrids. PMID: 25890199
Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening. PMID: 34495394
關(guān)鍵字： DNA-RNA,antibody,抗體;s9.6,經(jīng)典抗體;
武漢佰樂博生物（Biolab Reagents）由五位畢業(yè)于華中科技大學(xué)，華中農(nóng)業(yè)大學(xué)，武漢大學(xué)等知名高校，且具有二十年工作經(jīng)驗(yàn)的生命科學(xué)領(lǐng)域頂級(jí)研究者創(chuàng)立于2021年，憑借豐富的產(chǎn)品開發(fā)經(jīng)驗(yàn)，利用全球領(lǐng)先的技術(shù)平臺(tái)，引進(jìn)和整合全球高品質(zhì)的蛋白、抗體和試劑盒產(chǎn)品。目前，佰樂博生物作為法國AntibodySystem和ProteoGenix在亞洲的獨(dú)家總代理，提供近30,000種生命科學(xué)試劑，核心產(chǎn)品涵蓋蛋白、抗體和試劑盒，旨在為科研工作者提供專業(yè)、全面、可靠的試劑支持，推動(dòng)生命科學(xué)研究的深入發(fā)展。