中文名稱

Rho/Rac/Cdc42 激活劑 I

英文名字

Rho/Rac/Cdc42 Activator I

供應(yīng)商

Cytoskeleton

產(chǎn)品貨號(hào)

CN04-A

背景資料

該Rho/Rac/Cdc42 激活劑 I產(chǎn)品是Cytoskeleton品牌原裝進(jìn)口，Cytoskeleton品牌是細(xì)胞骨架研究專家，專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。 

產(chǎn)品描述

The G-switch? Direct line of small G-protein modulators have been developed with an emphasis on creating highly potent cell permeable reagents that directly target the Rho family of small G-proteins.  Our Direct Activator reagents are based on the catalytic domain of the bacterial CNF toxins, which are covalently attached to a proprietary cell penetrating moiety.  The Rho/Rac/Cdc42 Activator I (Cat# CN04) enters the cell and activates Rho GTPase isoforms by deamidating glutamine-63 of Rho and glutamine-61 of Rac and Cdc42 in their respective Switch II regions (1,2).  This modification converts glutamine-63 to glutamate, which blocks intrinsic and GAP stimulated GTPase activity resulting in constitutively active Rho (3).  The Rho/Rac/Cdc42 Direct Activator robustly increases the level of GTP bound RhoA, Rac1 and Cdc42 within 2-4 h after addition to the culture medium and thus provides a more rapid alternative to transfection based methods for introducing activators like GEFs into cells.  Moreover, the targeted action of the Rho Direct Activator makes it far more attractive tool for the study of Rho GTPase signaling than classic indirect activators (e.g. LPA, EGF, Bradykinin and Sphingosine-1-phosphate) that concomitantly activate other signaling pathways (e.g. Ras, PI3K and PLC).

產(chǎn)品形式

該Rho/Rac/Cdc42 激活劑 I產(chǎn)品是Cytoskeleton品牌的原裝進(jìn)口產(chǎn)品，具體的產(chǎn)品形式查看英文產(chǎn)品說明書。

保存建議

廠家推薦常溫運(yùn)輸，收到產(chǎn)品后建議保存于4°C。

其他

Cytoskeleton公司成立于1993年，專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。公司提供藥物篩選、信號(hào)轉(zhuǎn)導(dǎo)、蛋白質(zhì)轉(zhuǎn)錄后修飾（PTM）、細(xì)胞骨架研究相關(guān)的系列試劑盒和產(chǎn)品，尤其以細(xì)胞骨架相關(guān)研究見長，既能滿足于樣品較少的科學(xué)研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動(dòng)蛋白，小G蛋白，GAPs，GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務(wù)。