角蛋白15(CK15)重組蛋白
[ PROPERTIES ]
Residues: Val274~Leu399 (Accession # P19012),
with N-terminal His-Tag.
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.1
Predicted Molecular Mass: 16.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MG HH HHH HSGSEF - V LAEM RE QYE AMA EK N R RDV EAW F FS K TEE L NK EVAS
NTEMIQTSKT EITDLRRTMQ ELEIELQSQL SMKAGLENSL AETECRYATQ LQQIQGLIGG
LEAQLSELRC EMEAQNQEYK MLLDIKTRL
[ REFERENCES ]
1. Chong L.Y., et.al (2012) Breast Cancer Res. Treat. 132:143-151.
2. Sperling L.C., et.al (2011) J. Cutan. Pathol. 38:407-414.
3. Radoja N., et.al (2004) Mol. Cell. Biol. 24:3168-3179.
4. Whittock N.V., et.al (2000) Biochem. Biophys. Res. Commun. 267:462-465.
5. Waseem A.,et.al (1999) J. Invest. Dermatol. 112:362-369.
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關(guān)鍵字： KRT15;K15;CK15;角蛋白15(CK15)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
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