Ethos Biosciences的小鼠Nephrin ELISA試劑盒包含您檢測小鼠Nephrin所需的全部組分和試劑,最多可檢測40份重復(fù)的尿液或無細(xì)胞液體樣本(血漿、血清、細(xì)胞培養(yǎng)上清液等)。
該試劑盒由Exocell的腎臟病學(xué)專家開發(fā),并已更新以消除與白蛋白的交叉反應(yīng)性。Nephrin是一種在腎臟腎小球足細(xì)胞中表達的跨膜蛋白;在實驗動物和人類糖尿病的研究表明,Nephrin尿液排泄是腎小球濾過功能障礙或足細(xì)胞損傷的標(biāo)志。
作為一種間接競爭性ELISA,該檢測使用辣根過氧化物酶(HRP)標(biāo)記的抗體來檢測尿液中微量的Nephrin。小鼠Nephrin ELISA試劑盒包含一個96孔板,考慮到標(biāo)準(zhǔn)孔,一個試劑盒可用于分析80份樣本或40份重復(fù)樣本(推薦)。過夜孵育允許用戶在第一天準(zhǔn)備檢測,并在第二天評估數(shù)據(jù)。

艾美捷Ethos Biosciences-小鼠Nephrin ELISA試劑盒:
貨號:1037
所需樣本: 尿液,100 µL
檢測范圍: 0.031-2.0 µg/ml
精密度: 在有效范圍內(nèi)的樣本,批內(nèi)和批間精密度的變異系數(shù)(C.V.)小于10%。
特異性: 抗Nephrin抗體(兔源)可與多種哺乳動物物種(包括嚙齒動物和人類)發(fā)生反應(yīng)。
重量: 2磅
規(guī)格: 11 × 10 × 15英寸
Ethos Biosciences-小鼠Nephrin ELISA試劑盒組成:
- 一塊小鼠Nephrin檢測板
- 兩瓶EIA稀釋液
- 一瓶小鼠Nephrin標(biāo)準(zhǔn)品
- 一瓶抗小鼠Nephrin抗體
- 一瓶抗兔IgG-HRP偶聯(lián)物
- 顯色劑/終止液
- 說明書
文獻參考:
1. Putaala, H et al: The murine nephrin gene is specifically expressed in kidney, brain and pancreas: inactivation of the gene leads to massive proteinuria and death. Hum Mol Genet 10:1-8, 2001
2. Putaala, H. et al: Primary structure of mouse and rat nephrin cDNA and structure and expression of the mouse gene. J Am Soc Nephrol 11:991-1001, 2000
3. Holtzman, LB et al: Nephrin localizes to the slit pore of the glomerular epithelial cell. Kidney Int 56:1481-1491, 1999
4. Routsalainen, V et al: Nephrin is specifically located at the slit diaphragm of glomerular podocytes. Proc Natl Acad Sci USA 96:7962-7967, 1999
5. Holthofer, H et al: Nephrin localizes at the podocyte filtration area and is characteristically spliced in the human kidney. Am J Pathol 155:1681-1687, 1999
6. Kestila, M et al: Positionally cloned gene for novel glomerular protein – nephrin – is mutated in congenital nephrotic syndrome. Mol Cell 1:575-582, 1998
7. Cooper, ME et al: Role of nephrin in renal disease including diabetic nephropathy. Semin Nephrol 22:393-398, 2002
8. Pagtalunan, ME et al: Podocyte loss and progressive glomerular injury in type 2 diabetes. J Clin Invest 99:342-348, 1997
9. Aaltonen, P et al: Changes in the expression of nephrin gene and protein in experimental diabetic nephropathy. Lab Invest 81:1185-1190, 2001
10. Luimula, P et al: Nephrin in experimental glomerular disease.Kidney Int 58:1461-1468, 2000
11. Patari, S et al: Nephrinuria in diabetic nephropathy of type 1 diabetes. Diabetes 52:2969-2974, 2003.
艾美捷科技是Ethos Biosciences的中國代理商,為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。
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