1309444-75-4
| 中文名稱 | 1309444-75-4 |
|---|---|
| 中文同義詞 | 化合物 T11739;化合物 K145 |
| 英文名稱 | K145 |
| 英文同義詞 | K145;3-(2-Aminoethyl)-5-[3-(4-butoxyphenyl)propylidene]-2,4-thiazolidinedione;2,4-Thiazolidinedione, 3-(2-aminoethyl)-5-[3-(4-butoxyphenyl)propylidene]-;K145 ，K145 HCl salt;K145,K-145 |
| CAS號 | 1309444-75-4 |
| 分子式 | C18H24N2O3S |
| 分子量 | 348.46 |
| EINECS號 | |
| 相關(guān)類別 | |
| Mol文件 | 1309444-75-4.mol |
| 結(jié)構(gòu)式 |  |
1309444-75-4 性質(zhì)
| 溶解度 | 溶于二甲基亞砜 |
|---|---|
| 形態(tài) | 粉末 |
IC50: 4.3 μM (SphK2)
Ki: 6.4 μM (SphK2)
K145 (0-10 μM; 24-72 hours; U937 cells) treatment significantly inhibits the growth of U937 cells in a concentration-dependent manner.
K145 (10 μM; 24 hours; U937 cells) treatment significantly induces apoptosis in U937 cells.
K145 (4-8 μM; 3 hours; U937 cells) treatment decreases the phosphorylation of ERK and Akt.
Treatment with K145 (10 μM) causes a decrease of total cellular S1P without significant effects on ceramide levels.
Cell Viability Assay
| Cell Line: | U937 cells |
| Concentration: | 0 μM, 4 μM, 6 μM, 8 μM, 10 μM |
| Incubation Time: | 24 hours, 48 hours, 72 hours |
| Result: | Significantly inhibited the growth of U937 cells in a concentration-dependent manner. |
Apoptosis Analysis
| Cell Line: | U937 cells |
| Concentration: | 10 μM |
| Incubation Time: | 24 hours |
| Result: | Significantly induced apoptosis in U937 cells. |
Western Blot Analysis
| Cell Line: | U937 cells |
| Concentration: | 4 μM, 8 μM |
| Incubation Time: | 3 hours |
| Result: | Phosphorylated ERK and Akt were decreased. |
K145 (50 mg/kg; oral gavage; daily; for 15 days; BALB/c-nu mice) treatment significantly inhibits the growth of U937 tumors in nude mice.
| Animal Model: | BALB/c-nu mice injected with U937 cells |
| Dosage: | 50 mg/kg |
| Administration: | Oral gavage; daily; for 15 days |
| Result: | Oral gavage; daily; for 15 daysInhibited the growth of U937 tumors at 50 mg/kg dose and no apparent toxicity was observed. |