Monoclonal Anti-Phosphotyrosine-Agarose antibody produced in mouse has been used in:
immunoprecipitation experiments for affinity-purification of phosphotyrosine proteins
phosphotyrosine pulldown assays
PI3-kinase assay
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As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.
Biochem/physiol Actions
Tyrosine residue phosphorylation plays a key role in cell growth and differentiation.
