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Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.

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Beijing Boorsen Biotechnology Co., Ltd	400-901-9800 18611424007	cis@bioss.com.cn	China	9518	58
MedChemexpress LLC	021-58955995	sales@medchemexpress.cn	United States	4861	58
Shanghai Macklin Biochemical Co.,Ltd.	15221275939 15221275939	shenlinxing@macklin.cn	China	16166	55
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51456	80
Beijing Solarbio Science & Tecnology Co., Ltd.	010-50973186 4009686088	3193328036@qq.com	China	18338	68
Beijing Jin Ming Biotechnology Co., Ltd.	010-60605840 18892239720	psaitong@jm-bio.com	China	12306	58
Shanghai Zeye Biotechnology Co., Ltd.	021-61998551 13122364865	sale1@shzysw.net	China	9772	58
Absin Bioscience Inc.	021-38015121 15000105423	wulan@absin.cn	China	24731	58
Shanghai Fusheng Industrial Co., LTD	021-52961053 13524666654	3004902811@qq.com	China	9724	58
Shanghai Sig Biotechnology Co., LTD	021-57810052 18930344717	2089316240@qq.com	China	9395	58

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