ChemicalBook >?? ???? >APN-Azide 95%
APN-Azide 95%
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APN-Azide 95% ??
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- 100°C
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- 2-8°C
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| ????(UN No.) | UN1325 - class 4.1 - PG 2 - Flammable solids, organic, n.o.s., HI: all |
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APN-Azide 95% C??? ??, ??, ??
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APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.Standard protein labeling procedure (cysteine labeling)
1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
3. Incubate at room temperature for 2 hours.
4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.
5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.
*Note: avoid thiol-containing buffers.
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