?? MSDS

Nisin

?? C??? ??, ??, ??

????

? (1) ? : ? ?? 5.0g? ??? ??????? ?? ?????????????? ?? ??? ?, ? ?? 1.0ppm ????? ??.

??(2) ?? : ? ??? ?????? ?? ??? ?, ? ?? 1.3ppm ????? ??.

??(3) ?? : ? ??? ?????? ?? ??? ?, ? ?? 1.0ppm ????? ??.

??(4) ??? : ? ??? 「??? ?? ? ??」 ?????? ?????? ? ???(?????) ???? ?? ??? ?, 1g? 10CFU ????? ??.

??(5) ??? : ? ??? 「??? ?? ? ??」 ?????? ?????? ? ???? ?? ??? ?, 25g?? ??(-) ??? ??.

??(6) ???? : ? ??? 「??? ?? ? ??」 ?????? ?????? ? ????? ?? ??? ?, 25g?? ??(-) ??? ??.

??(7) ???? : ? ??? 「??? ?? ? ??」 ?????? ?????? ? ????? ?? ??? ?, ?? 1g? 30 ????? ??.

????

? (1) ? ??? : ? ?? 100mg? ??? ??? ? ?? ????? ????? ?? 0.02N ??? ?????. ? ??? 5?? ?? ? ??? ? ???? ?? ????? ???. ? ?, ????? ?? ??? ??? ??? ???? ??? ??. ?? ??? ????? ????? 100±5%??.? ????? 5N ????????? ???? pH 11? ??? ? 65℃?? 30?? ??? ?? ???. ??? ??? ?? pH 2.0?? ?? ? ???? ?? ?? ????? ???. ? ??? ?? ??? ????? ??? ??????? ????.

??(2) ???? ??? ?? Lactococcus lactis? ?? : Lactococcus lactis(ATCC 11454, NCIBM 8586)? ?? ???? ?? 30℃, 18?? ?? ????. Litmus milk 100mL? ?? ?? ??? ????? 121℃, 15?? ????. ??? Litmus milk? ?? 0.1g? ?? ? ???? ???? 2?? ??? ? L. lactis ??? 0.1mL? ?? 30℃, 24?? ?? ????. ? ?, L. lactis? ? ????(? 1,000 IU/mL)?? ??? ?? ????.

???

???? ? : Bacteriological Peptone 10g, Beef Extract 3g, Sodium Chloride 3g, Autolyzed Yeast　(Yeast Extract) 1.5g, Brown Sugar 1g ? Agar 15g? ? 1,000mL? ?? 121℃?? 15?? ????. ??? ??? ??? ?? ??? ? ??. ??? ??? ??? ?? ? 50℃? ?? ? Tween 20(polyoxyethylene sorbitan monolaurate)? ?(1 : 1)? ??? 2%? ??? ??? ?? 48℃?? 20～30? ?? ??? ? ????.

? ???? : Micrococcus luteus(ATCC 10240, NCIMB 8166)? ????? ???? 30℃?? 48?? ????. ????? 4℃?? 14??? ???? ??? ? ??. ???? ???? ??????? 7mL? ???? ???.

???? ???? : ?????(1,000 IU/mg) 1,00mg? ??? ?? 0.02N ?? 80mL? ???? ? ???? 2?? ??? ?? 0.02N ??? ??? 100mL? ????(1,000 IU/mL). ????? 4℃?? 7?? ??????.

???? ???? : ?? ???? ?? 0.5, 1.0, 2.5, 5.0 ? 10.0mL? ??? ??? ? 1,000mL ??????? ?? 0.02N ??? ??? 1,000mL? ??(0.5, 1.0, 2.5, 5.0 10.0 IU/mL). ? ????? ??? ????.?

?????? ?? : ???? ???? ???????? ??(1 : 10) ?? ??? ????. ? ?? 2mL? ??? 48℃? ??? ?? ?? 100mL? ???. ??? ??? 5?? ?????? ?? 3～4mm ??(? 15mL)? ?? ?? ? ?????? ??? 4℃?? 1?? ????. ??? Agar ????? 30mm ???? ?? 8～9mm? ?? 4?? ???. ?? ??? ????? ???? ??? ?? ??. ????? ??? ???? ??? 0.5, 1.0, 2.5, 5.0, 10.0 IU/mL? ?? ???? ?? 0.2mL? ???. ? ? ??? ?????? ? ??? ????? ???. ????? ??? ?? 30℃?? ??? ??? ? ????? ?? ??? ??? 0.1mm?? ???? ????. ???? ??? ?? ?? ???? ??? ?? ???? ???? ???? ????.

?????? : 0.02N ?? 80mL? ?? 100mL ??????? ?? 100mg? ???? ???? 2?? ??? ? 0.02N ??? ??? 100mL? ??? ?? 0.02N ???? 200?(1:200)? ????. ?? ??? ????? ?? Agar ???? ?? ?? 4?? ??? 4? ???? ?? 0.2mL? ?? ? ????? ??? ?? 30℃?? ??? ??? ?? ???? ????. ????? ?? ?? ???? ??? ???? ????.

??

? ? ??? Lancefield group N? ??? Lactococcus lactis (Streptococcus Lactis)? ??? ??????? ?????? ??? ?????.

??? ??

Crystals from ethanol. Derived from Streptococcus lactis Lancefield Group N.

??

Nisin is an antimicrobial agent derived from pure culture fermenta- tions of certain strains of streptococcus lactis lancefield group n. nisin preparation contains nisin, a group of related peptides with antibiotic activity. it is used to inhibit the outgrowth of clostridium botulinum spores and toxin formation in pasteurized cheese spreads and pasteurized process cheese spreads; pasteurized cheese spread with fruits, vegetables, or meats; and pasteurized process cheese spread with fruits, vegetables, or meats.

??

ChEBI: A type-A lantibiotic containing 34 amino acid residues (including lanthionine (Lan), methyllanthionine (MeLan), didehydroalanine (Dha) and didehydroaminobutyric acid (Dhb)) and five thioether bridges. It is obtained by fermentation of the bacterium L ctococcus lactis and shows particular activity against Clostridium botulinum. It is used in the production of various processed foods to suppress Gram-positive spoilage and pathogenic bacteria and so extend shelf life.

???

Although Nisin E234 generally regarded as a very safe and effective supplement, there can be some minor side effects. Possible side effects: pruritus, nausea, and flushing. Other side effects include: pruritus, skin rash, and vomiting.

Purification Methods

This polypeptide from S. lactis is purified by crystallisation from 80% (v/v) EtOH and by countercurrent distribution. The synthetic polypeptide antibiotic can also be purified by preparative HPLC and assayed by HPLC on a Nucleosil 3007C18 (6 x 250mm) column using a MeCN—0.01M HCl gradient (30-50%), at 2%/minute, and flow rate of 1.5mL/minute to give a retention time of 8.1 minutes; or MeCN—0.3M guanidine-HCl gradient (30-50%), at 2%/minute, and flow rate of 1.5ml/minute to give a retention time of 10.9minutes. FAB-MS gave the pseudomolecular ion m/z at 3352.7 (M + H)+. It is soluble in dilute acid and is stable even on boiling. [Berridge et al. Biochem J 52 529 1952, synthesis by Fukase et al. Tetrahedron Lett 29 795 1988.]

?? ?? ?? ? ???

???

?? ??

?? ?? ??

???	??	???	??	?? ?	??
Nanjing Sky Hope Tongyuan Biological Engineering Co., Ltd.	+86-0086-025-69916489 +86-18852044786	tongyuansales@vip.sina.com	China	323	58
Hengshui Haoye Co.,Ltd.	+86-0318-2102300 +8618632882519	hy@haoyecom.cn	China	307	58
BOC Sciences	16314854226; +16314854226	inquiry@bocsci.com	United States	19654	58
Across Biotech Jinan Co LTD	+8613031735486	frank@acrossbiotech.com	China	105	58
Hebei Chuanghai Biotechnology Co., Ltd	+8615531157085	abby@chuanghaibio.com	China	8808	58
Hebei Mujin Biotechnology Co.,Ltd	+86 13288715578 +8613288715578	sales@hbmojin.com	China	12809	58
Hebei Chuanghai Biotechnology Co,.LTD	+86-13131129325	sales1@chuanghaibio.com	China	5868	58
Hebei Kingfiner Technology Development Co.Ltd	+86-15532196582 +86-15373005021	lisa@kingfinertech.com	China	3005	58
Hebei Xinsheng New Material Technology Co., LTD.	+86-16632316109	xinshengkeji@xsmaterial.com	China	1085	58
airuikechemical co., ltd.	+86-18353166132 +86-18353166132	sales02@airuikechemical.com	China	983	58

?? ?? ??: