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ChemicalBook >> CAS DataBase List >>WST-8

WST-8

CAS No.
193149-74-5
Chemical Name:
WST-8
Synonyms
2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt;WST-8;GLT008;CS-2725;fophenyL;WST8;WST 8;WST-8 sodium;)-5-(2,4-disuL;ium Sodium SaL;CAS:193149-74-5
CBNumber:
CB4547211
Molecular Formula:
C20H15N6NaO11S2
Molecular Weight:
602.48
MDL Number:
MFCD09264687
MOL File:
193149-74-5.mol
MSDS File:
SDS
Last updated:2025-02-27 17:19:27

WST-8 Properties

storage temp. under inert gas (nitrogen or Argon) at 2-8°C
solubility DMSO: 1 mg/ml; PBS (pH 7.2): 5 mg/ml
form A crystalline solid
color White to yellow

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS05
Signal word  Danger
Hazard statements  H314
Precautionary statements  P260-P264-P280-P301+P330+P331-P303+P361+P353-P363-P304+P340-P310-P321-P305+P351+P338-P405-P501
NFPA 704
0
3 0

WST-8 price More Price(17)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Cayman Chemical 18721 WST-8 ≥98% 193149-74-5 10mg $33 2024-03-01 Buy
Cayman Chemical 18721 WST-8 ≥98% 193149-74-5 50mg $112 2024-03-01 Buy
Cayman Chemical 18721 WST-8 ≥98% 193149-74-5 100mg $160 2024-03-01 Buy
Cayman Chemical 18721 WST-8 ≥98% 193149-74-5 500mg $443 2024-03-01 Buy
TRC W500038 WST-8 193149-74-5 100mg $250 2021-12-16 Buy
Product number Packaging Price Buy
18721 10mg $33 Buy
18721 50mg $112 Buy
18721 100mg $160 Buy
18721 500mg $443 Buy
W500038 100mg $250 Buy

WST-8 Chemical Properties,Uses,Production

Description

WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt), a highly stable and water-soluble WST, is utilized incell Counting Kit-8 (CCK-8).It is more sensitive than WST-1 particularly at neutralpH. Because of the electron mediator, 1-methoxy PMSin this kit is highly stable, and CCK-8 is stable for at least six months at the room temperature and for one year at 0-5°C (asshown in Figure 1).
WST-8
Advantages: WST-8 is not cell permeable, which results in low cytotoxicity. Therefore,after the assay, it is possible to continue further experiments using the same cells.Furthermore, it produces the water-soluble formazan upon cellular reduction，which would provide an additional advantage to the method by allowing a simpler assay procedure and not required an extra step to dissolve the formazan.
Disadvantages: An important consideration is that reduction of assay substrates isimpacted by changes in intracellular metabolic activity that has no direct effect on over-all cell viability.

Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing the highly water-soluble tetrazolium salt WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2Htetrazolium,monosodium salt] (Patent No. WO97/38985) produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a onebottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellowcolored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Uses

A highly sensitive tetrazolium reagent that produces a water-soluble formazan (orange color that absorbs at 460 nm) in NADH. It is newer generation formazan-based dyes which release the converted prod uct into the medium in a soluble form allowing for a non-destructive determination of viability enabling the cells to be subject to further investigations.

Uses

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.

Biotechnological Applications

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful as a cell viability indicator in cell proliferation assays.

WST-8 Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 281)Suppliers
Supplier Tel Email Country ProdList Advantage
ShanDong Look Chemical Co.,Ltd.
+8617653113219 sales01@sdlookchemical.com China 2737 58
Shandong Huisheng Import & Export Co., Ltd. 		+86-13176845580 +86-13176845580 da@zhongda-biotech.com China 232 58
Anhui Zhongda Biotechnology Co., Ltd 		+8619956560829 justine@zhongda-biotech.com China 286 58
Anhui Ruihan Technology Co., Ltd 		+8617756083858 daisy@anhuiruihan.com China 973 58
Henan Fengda Chemical Co., Ltd 		+86-371-86557731 +86-13613820652 info@fdachem.com China 20244 58
Shandong Deshang Chemical Co., Ltd. 		+86-0531-8875-2665 +8613153039501 info@deshangchem.com China 662 58
Hebei Junhua Import and Export Co., LTD 		+86-18503288844 +86-18503288844 junhua1@hb-junhua.com China 977 58
Henan Tianfu Chemical Co.,Ltd. 		+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21631 55
ATK CHEMICAL COMPANY LIMITED 		+undefined-21-51877795 ivan@atkchemical.com China 33024 60
Jilin Chinese Academy of Sciences - Yanshen Technology Co., Ltd. 		0431-80514535 13634302652 Extension@chemextension.com CHINA 967 58

View Lastest Price from WST-8 manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
WST-8 pictures 2025-03-31 WST-8
193149-74-5
 US $0.00 / kg 1kg 97% 1000 Hebei Junhua Import and Export Co., LTD
WST-8 pictures 2025-02-13 WST-8
193149-74-5
 US $10.00-5.00 / kg 1kg 99% 10 tons Shandong Deshang Chemical Co., Ltd.
WST-8 pictures 2024-11-19 WST-8
193149-74-5
 US $50.00-109.00 / mg 99.93% 10g TargetMol Chemicals Inc.
  • WST-8 pictures
  • WST-8
    193149-74-5
  • US $0.00 / kg
  • 97%
  • Hebei Junhua Import and Export Co., LTD
  • WST-8 pictures
  • WST-8
    193149-74-5
  • US $10.00-5.00 / kg
  • 99%
  • Shandong Deshang Chemical Co., Ltd.
  • WST-8 pictures
  • WST-8
    193149-74-5
  • US $50.00-109.00 / mg
  • 99.93%
  • TargetMol Chemicals Inc.

WST-8 Spectrum

5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2h-tetrazolium inner salt sodium salt (1:1) 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt Cell Proliferation Assay 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophen GLT008 5-(2,4-Disulfophenyl)-3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazoliuM Inner Salt SodiuM Salt 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium Sodium 4-[2-(2-methoxy-4-nitro-phenyl)-3-(4-nitrophenyl)tetrazol-3-ium-5-yl]benzene-1,3-disulfonate 2-(2-Methoxy-4-nitrophenyl) -2H-tetrazolium Sodium Salt -5-(2,4-disulfophenyl) Water-soluble tetrazoliuM -8 WST-8 SODIUM;4-[2-(2-METHOXY-4-NITROPHENYL)-3-(4-NITROPHENYL)TETRAZOL-2-IUM-5-YL]BENZENE-1,3-DISULFONATE WST8;WST 8 CS-2725 WST-8 sodium 4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate sodium 4-(2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate ReadiUse WST-8 *50 mM aqueous solution* )-5-(2,4-disuL fophenyL ium Sodium SaL )-3-(4-nitrophenyL 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt Water soluble four azole nitrogen 2-(3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-5-sulfobenzenesulfonate, sodium salt WST-8, (2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium Sodium Salt) WST-8,inhibit,Inhibitor,WST 8 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt(WST-8) 2H-Tetrazolium, 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-, inner salt, sodium salt (1:1) 2-(3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-5-sulfobenzenesulfonate CAS:193149-74-5 193149-74-5 C20H13N6NaO11S2 Heterocycles Intermediates & Fine Chemicals Labeling and Diagnostics Reagents Pharmaceuticals Aromatics