2-[Bis(2-hydroxyethyl)amino]ethansulfonsure
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2-[Bis(2-hydroxyethyl)amino]ethansulfonsure Eigenschaften
- Schmelzpunkt:
- 150-155 °C
- Dichte
- 1.210 (estimate)
- Dampfdruck
- 0.001Pa at 25℃
- Brechungsindex
- 1.5500 (estimate)
- storage temp.
- room temp
- L?slichkeit
- H2O: 1 M at 20 °C, clear, colorless
- Aggregatzustand
- Powder
- Farbe
- White
- PH
- 2.5-5.0 (25℃, 1M in H2O)
- pka
- 7.1(at 25℃) 7.15(at 20℃)
- S?ure-Base-Indikators(pH-Indikatoren)
- 6.4 - 7.8
- Wasserl?slichkeit
- Soluble
- maximale Wellenl?nge (λmax)
- λ: 260 nm Amax: 0.095
λ: 280 nm Amax: 0.080
- BRN
- 1781572
- LogP
- -3.59 at 26℃
- CAS Datenbank
- 10191-18-1(CAS DataBase Reference)
- NIST chemische Informationen
- N,n-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid(10191-18-1)
- EPA chemische Informationen
- Ethanesulfonic acid, 2-[bis(2-hydroxyethyl)amino]- (10191-18-1)
Sicherheit
- Risiko- und Sicherheitserkl?rung
- Gefahreninformationscode (GHS)
Kennzeichnung gef?hrlicher | Xi | ||
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R-S?tze: | 36/37/38 | ||
S-S?tze: | 26 | ||
WGK Germany | 3 | ||
RTECS-Nr. | KI7363500 | ||
TSCA | Yes | ||
HS Code | 29221980 |
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Sicherheit |
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2-[Bis(2-hydroxyethyl)amino]ethansulfonsure Chemische Eigenschaften,Einsatz,Produktion Methoden
R-S?tze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.S-S?tze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.Beschreibung
BES is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is one of the "Good" buffers developed in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of BES and its physical properties.A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. An investigation has been published on the interaction of BES and other amine buffers with DNA and the measurement of these complexes by free solution capillary electrophoresis.
The effect of BES and other zwitterionic buffers on the bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.
Chemische Eigenschaften
White/clear cryst. powderVerwenden
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C. Absorbance (100 mM, 260 nm):≤0.05.BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
Application
BES, or N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, can be used to study molecular biology, biological buffers, molecular biology reagents and zwitterionic compounds. BES has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES has also been used to determine that saline and buffers minimize the action of interfering factors in the bacterial endotoxins test.BES is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
synthetische
First, sodium vinyl sulfonate and diethanolamine synthesize N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium by addition reaction. Through the strong acid ion exchange resin sodium ions, the eluent concentrated after adding methanol crystallization. Finally, BES is obtained after precipitation.Allgemeine Beschreibung
BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.l?uterung methode
Crystallise BES from aqueous EtOH. [Beilstein 4 IV 3290.]2-[Bis(2-hydroxyethyl)amino]ethansulfonsure Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
2-[Bis(2-hydroxyethyl)amino]ethansulfonsure Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.
Global( 411)Lieferanten
Firmenname | Telefon | Land | Produktkatalog | Edge Rate | |
---|---|---|---|---|---|
Hebei Mojin Biotechnology Co., Ltd | +86 13288715578 +8613288715578 |
sales@hbmojin.com | China | 12825 | 58 |
Hebei Weibang Biotechnology Co., Ltd | +8617732866630 |
bess@weibangbio.com | China | 18275 | 58 |
Henan Bao Enluo International TradeCo.,LTD | +86-17331933971 +86-17331933971 |
deasea125996@gmail.com | China | 2472 | 58 |
Watson Biotechnology Co.,Ltd | +86-18186686046 +86-18186686046 |
sales01@watsonbiotech.cn | China | 5804 | 58 |
Capot Chemical Co.,Ltd. | +86-(0)57185586718 +86-13336195806 |
sales@capot.com | China | 29791 | 60 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21639 | 55 |
Shanghai Time Chemicals CO., Ltd. | +86-021-57951555 +8617317452075 |
jack.li@time-chemicals.com | China | 1803 | 55 |
career henan chemical co | +86-0371-86658258 +8613203830695 |
sales@coreychem.com | China | 29886 | 58 |
Hubei Jusheng Technology Co.,Ltd. | 18871490254 |
linda@hubeijusheng.com | CHINA | 28172 | 58 |
Shanghai Longyu Biotechnology Co., Ltd. | +8619521488211 |
info@longyupharma.com | China | 2524 | 58 |
10191-18-1(2-[Bis(2-hydroxyethyl)amino]ethansulfonsure)Verwandte Suche:
Ethansulfonsure
2-Butoxyethylstearat
2-(Diethylamino)ethanthiol
N-Ethyldiethanolamin
Taurin
Diethanolamin
Captamin
2,2'-Methyliminodiethanol
2-[Bis(2-hydroxyethyl)amino]ethansulfonsure
N-Methyltaurin
- BES ULTRAPURE
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulphonic acid >99%
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid [Good's buffer component for biological research]
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
- Bes, 99+%, for biochemistry
- BES, for biochemistry
- 2-[N,N-Bis(2-hydroxyethyl)amino]ethanesulfonic acid (BES)
- Ethanesulfonic acid, 2-[bis(2-hydroxyethyl)amino]-
- n,n-bis(2-hydroxyethyl)-taurin
- Taurine, N,N-bis(2-hydroxyethyl)-
- N,N-BIS(HYDROXYETHYL)-2-AMINOETHANESULFONIC ACID
- N,N-BIS(2-HYDROXYETHYL)-2-AMINOETHANESULPHONIC ACID
- 2-[BIS(2-HYDROXYETHYL)AMINO]ETHANESULFONIC ACID
- BES N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid
- BES, Free Acid, ULTROL Grade
- BES, Molecular Biology Grade N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid, Molecular Biology Grade
- 2-[bis(2-hydroxyethyl)amino]ethanesulphonic acid
- BES FREE ACID MOLECULAR BIOLOGY REAGENT
- BES, SIGMAULTRA
- BES, BIOTECHNOLOGY PERFORMANCE CERTIFIED, CELL CULTURE TESTED
- BIS(2-HYDROXYETHYL)-2-AMINOETHANESULPHONIC ACID
- BES
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid(BES)
- Nsc166667
- N,N-Bis(2-hydroxyeth
- BES, for biocheMistry, 99+% 100GR
- BES Vetec(TM) reagent grade, >=99%
- BES buffer free acid
- BES, BIOPERFORMANCE CERTIFIED&
- BES, Free Acid, ULTROL
- N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, free acid
- 2-(bis(2-hydroxyethyl)amino)-ethanesulfonicaci
- 2-[bis(2-hydroxyethyl)amino]-ethanesulfonicaci
- bes(bufferingagent)
- 2-[N,N-BIS(2-HYDROXYETHYL)AMINO]ETHANESULFONIC ACID
- BesN,N-Bis(2-Hydroxyethyl)-2-AminoethanesulfonicAcid
- BES, for biochemistry, 99+%
- BES,BIOLOGICALBUFFER
- N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
- BES, ≥99%(T)
- BES, ≥99.5%(T)
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid [Good's buffer component for biological research]>
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulphonic acid, 99%, reagent grade
- N,N-Bis(2-hydroxyethyl)-2-aminoethanesulphonic acid, 99%, for bio
- 2-[Bis(2-hydroxyethyl)amino]ethansulfonsure
- 99% purity BES
- abs9128
- N. N-di (2-hydroxyethyl) - 2-aminoethanesulfonic Acid
- BES Buffer extrapure, 99%
- BES Buffer for cell culture, 99%, Endotoxin (BET) 0.05EU/mg
- N,N-BIS(2-HYDROXYETHYL)-2-AMINOETHANESULFONIC ACID
- N,N-Bis(2-hydroxyethyl)taurine
- 2-(diethanolamine) ethanesulfonic acid
- BES buffer {N,N-Bis[2-hydroxyethyl]-2-aminoethanesulphonic acid}
- BES Buffer 99% For Biochemistry
- 10191-18-1
- 191-18-1
- 10191-81-1