ANTI-MAP KINASE, ACTIVATED (DIPHOSPHORYLATED ERK-1&2) ANTIBODY, MOUSE MONOCLONAL Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
The antibody may be used for immunoblotting applications at a working concentration of 0.5-1.0 μg/mL in cultured cells as well as tissue extracts. A working dilution of 1:80-1:100 is suitable for immunohistochemistry (formalin and formaldehyde-fixed sections). The antibody may also be used for immunocytochemistry, immunoprecipitation, indirect ELISA and protein microarray.
Monoclonal Anti-MAP Kinase, activated (Diphosphorylated ERK-1&2) antibody produced in mouse has been used in detection of
- ERK1/2 in cardiac myocytes using immunofluorescence microscopy
- ERK1/2 in human eosinophils using western blotting
- ERK in human bronchial epithelial cells using protein array
- ERK proteins in human liver cell lines using immunofluorescence assay and western blot analysis
- ERK1/2 in human embryonic kidney cells by immunoprecipitation
Allgemeine Beschreibung
Mitogen-activated protein kinase (MAPK) are also known as the extracellular signal-regulated kinase (ERKs), they exist in two isoforms namely, ERK1 (MAPK3) and ERK2 (MAPK1). ERK2 is mapped to human chromosome 22q11.2. ERK1 is located on human chromosome 16p11.2.
Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the MAPK-YT hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic phosphorylated peptide containing amino acids corresponding to the phosphorylated form of ERK-activation loop, conjugated to KLH. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2.
ANTI-MAP KINASE, ACTIVATED (DIPHOSPHORYLATED ERK-1&2) ANTIBODY, MOUSE MONOCLONAL Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
