DIG RNA Labeling Kit (SP6/T7)
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- CAS-Nr.
- Englisch Name:
- DIG RNA Labeling Kit (SP6/T7)
- Synonyma:
- DIG RNA Labeling Kit (SP6/T7)
- CBNumber:
- CB72541649
- Summenformel:
- Molgewicht:
- 0
- MOL-Datei:
- Mol file
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DIG RNA Labeling Kit (SP6/T7) Eigenschaften
Sicherheit
- Risiko- und Sicherheitserkl?rung
- Gefahreninformationscode (GHS)
DIG RNA Labeling Kit (SP6/T7) Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
For RNA labeling with DIG-11-UTP by
in vitro transcription with SP6 and T7 RNA Polymerases. DIG-labeled "run off" transcripts are synthesized with high efficiency and can be used in a variety of hybridization techniques:
- Northern blots
- Southern blots
- In situ hybridizations
- Plaque or colony lifts
- RNase protection experiments
Due to highly specific and sensitive detection systems, DIG-labeled probes can be used for single-copy gene detection in 1μg total human DNA.
Note: Since the linkage between DIG and UTP is resistant to alkali, DIG-labeled RNA can be fragmented by alkaline treatment. Slightly reducing the size of the DIG-labeled RNA probe may make it more suitable for certain applications in
in situ hybridization.
Allgemeine Beschreibung
Kit for the labeling of RNA with digoxigenin-UTP by
in vitro transcription with SP6 and T7 polymerases. By this method, single-stranded RNA probes of known length are produced, which can be used in a variety of hybridization techniques.
DIG RNA Labeling Kit (SP6/T7) Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
DIG RNA Labeling Kit (SP6/T7) Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.
Global( 2)Lieferanten
- DIG RNA Labeling Kit (SP6/T7)
