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CATALASE ASSAY KIT

CATALASE ASSAY KIT Struktur
CAS-Nr.
Englisch Name:
CATALASE ASSAY KIT
Synonyma:
CATALASE ASSAY KIT
CBNumber:
CB6509833
Summenformel:
Molgewicht:
0
MOL-Datei:
Mol file

CATALASE ASSAY KIT Eigenschaften

storage temp. 
2-8°C
Specific Activity
0.25 - 4nmol/min⋅mL

Sicherheit

CATALASE ASSAY KIT Chemische Eigenschaften,Einsatz,Produktion Methoden

Beschreibung

Catalase (EC 1.11.1.6; 2H2O2 oxidoreductase) is an ubiquitous antioxidant enzyme that is present in most aerobic cells. Catalase (CAT) is involved in the detoxification of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), which is a toxic product of both normal aerobic metabolism and pathogenic ROS production. This enzyme catalyzes the conversion of two molecules of H2O2 to molecular oxygen and two molecules of water (catalytic activity). CAT also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. While the aliphatic alcohols serve as specific substrates for CAT, other enzymes with peroxidatic activity do not utilize these substrates. In humans, the highest levels of catalase are found in liver, kidney, and erythrocytes, where it is believed to account for the majority of hydrogen peroxide decomposition. The Cayman Chemical Catalase Assay Kit utilizes the peroxidatic function of CAT for determination of enzyme activity. The method is based on the reaction of the enzyme with methanol in the presence of an optimal concentration of H2O2. The formaldehyde produced is measured spectrophotometrically with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald) as the chromogen. Purpald specifically forms a bicyclic heterocycle with aldehydes, which upon oxidation changes from colorless to a purple color. The assay can be used to measure CAT activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates.

CATALASE ASSAY KIT Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


CATALASE ASSAY KIT Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Global( 12)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
amyjetsci 027-59626688 18771149750
sales@amyjet.com China 3002 58
4008-099-669
23419001name@qq.com CHINA 73043 58
Cayman Chemical Company 800-3649897
Caymanname@qq.com China 21643 58
Merck KGaA (+86) 21 2033 8288
sigmaname@qq.com CHINA 292394 58

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