Cell Proliferation Kit I (MTT)
|
|
|
- CAS-Nr.
- Englisch Name:
- Cell Proliferation Kit I (MTT)
- Synonyma:
- Cell Proliferation Kit I (MTT)
- CBNumber:
- CB14669901
- Summenformel:
- Molgewicht:
- 0
- MOL-Datei:
- Mol file
|
Cell Proliferation Kit I (MTT) Eigenschaften
- storage temp.
- -20°C
- Aggregatzustand
- liquid
- maximale Wellenl?nge (λmax)
- 550-600 nm
Sicherheit
- Risiko- und Sicherheitserkl?rung
- Gefahreninformationscode (GHS)
| Bildanzeige (GHS) |
|
| Alarmwort |
Achtung |
| Gefahrenhinweise |
| Code |
Gefahrenhinweise |
Gefahrenklasse |
Abteilung |
Alarmwort |
Symbol |
P-Code |
| H318 |
Verursacht schwere Augensch?den. |
Schwere Augensch?digung |
Kategorie 1 |
Achtung |
![GHS hazard pictograms]() src="/GHS05.jpg" width="20" height="20" /> |
P280, P305+P351+P338, P310 |
|
| Sicherheit |
| P280 |
Schutzhandschuhe/Schutzkleidung/Augenschutz tragen. |
|
Cell Proliferation Kit I (MTT) Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
The Cell Proliferation Kit I (MTT) is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. It can be used for:
- Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients
- Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
- Assessment of growth-inhibitory antibodies and physiological mediators
- Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth
Allgemeine Beschreibung
The Cell Proliferation Kit I (MTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.
Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate.
MTT was the first tetrazolium salt described. It is cleaved to formazan by enzymes of the endoplasmic reticulum. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.
Colorimetric assay (MTT based) for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity.
Cell Proliferation Kit I (MTT) Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
Cell Proliferation Kit I (MTT) Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.
Global( 14)Lieferanten
- Cell Proliferation Kit I (MTT)
