Anti-WRN antibody, Mouse monoclonal Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Anti-WRN antibody has been used in
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunohistochemistry
- western blotting
Allgemeine Beschreibung
Anti-WRN antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 195C produced by the fusion of mouse myeloma cells (p3-NS1/Ag4-1) and splenocytes from BALB/c mice immunized with a recombinant fusion protein fragment of human WRN. Werner syndrome ATP-dependent helicase (WRN) is located on chromosome 8 and is 167 kDa. The WRN protein belongs to the RecQ helicase family of DNA helicases.
Biochem/physiol Actions
Werner syndrome ATP-dependent helicase (WRN) protein has been demonstrated to possess three known catalytic activities: 3′-5′ helicase, exonuclease and ATPase activities. The C-terminal region of the protein contains a nuclear localization signal (NLS), thus targeting WRN protein to the nucleus, nucleolus and nucleoplasmic foci. The involvement of WRN in multiple DNA metabolic process suggests its function as a tumor suppressor. Mutations in the WRN protein results in Werner syndrome (WS). The epigenetic inactivation of WRN leads to loss of WRN-exonuclease activity, resulting in increased chromosomal instability and hypersensitivity to chemotherapeutic drugs.
Anti-WRN antibody, Mouse monoclonal Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
