Carboanhydrase Chemische Eigenschaften,Einsatz,Produktion Methoden
Industrielle Verwendung
Carboanhydrases are enzymes that catalyse the hydrolysis of carbon dioxide. These enzymes are involved in many biological
processes such as photosynthesis (CO
2 uptake), respiration (CO
2 release) and pH control.
The human carboanhydrase, form II(c), consists of 259 amino acids with a molecular weight of around 30 kDa. The
catalytic site contains a Zn
2+ ion which is coordinated by three neutral histidine (His) residues and a water
molecule. The water molecule is believed to be important for structural reasons and enzymatic functionality
.In a first step (i), a proton
is transferred to His64 from the coordinated water molecule. In a second step (ii), a buffer molecule (B) coordinates
this proton and transports it away from the active site. The remaining hydroxide ligand reacts quickly
and forms a transition state via hydrogen bonding with CO
2 (iii). Following some more transformations,
HCO
3- is released as it is replaced by another molecule of water (iv) and (v).
Carboanhydrase Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
