93718-83-3
中文名稱
NSC 95397
英文名稱
NSC 95397
CAS
93718-83-3
分子式
C14H14O4S2
分子量
310.39
MOL 文件
93718-83-3.mol
更新日期
2024/10/22 09:47:18
93718-83-3 結(jié)構(gòu)式
基本信息
中文別名
化合物NSC 953972,3-雙(2-羥基乙硫基)-1,4-萘二酮
英文別名
NSC 95397Cdc25 Inhibitor IV, NSC 95397
2,3-BIS[(2-HYDROXYETHYL)THIO]-1,4-NAPHTHOQUINONE
2,3-BIS(2-HYDROXYETHYLTHIO)-1,4-NAPHTHALENEDIONE
2,3-BIS[(2-HYDROXYETHYL)THIOL]-1,4-NAPHTHOQUINONE
1,4-Naphthalenedione, 2,3-bis[(2-hydroxyethyl)thio]-
Cdc25 Inhibitor IV, NSC 95397 - CAS 93718-83-3 - Calbiochem
物理化學(xué)性質(zhì)
儲存條件-20°C
溶解度DMSO: 16 mg/mL
溶解度二甲基亞砜：16 毫克/毫升
形態(tài)橙色固體
顏色orange, solid
穩(wěn)定性自購買之日起 1 年內(nèi)保持穩(wěn)定。 DMSO 或乙醇溶液可在 -20°C 下保存長達(dá) 1 個月。
NSC 95397價格(試劑級)
| 報價日期 | 產(chǎn)品編號 | 產(chǎn)品名稱 | CAS號 | 包裝 | 價格 |
| 2024/08/19 | HY-108543 | NSC 95397 NSC 95397 | 93718-83-3 | 5mg | 1200元 |
| 2024/08/19 | HY-108543 | NSC 95397 NSC 95397 | 93718-83-3 | 10mM * 1mLin DMSO | 1320元 |
| 2024/08/19 | HY-108543 | NSC 95397 NSC 95397 | 93718-83-3 | 10mg | 1800元 |
常見問題列表
生物活性
NSC 95397 是一種有效的、選擇性 Cdc25 雙特異性磷酸酶抑制劑，對于 Cdc25A、Cdc25B 和 Cdc25C 的Ki值分別為32 nM、96 nM 和 40 nM。NSC 95397 對 hCdc25A、hCdc25C 和 hCdc25B 的IC50值分別為22.3 nM、56.9 nM和125 nM。NSC 95397 抑制 mitogen-activated protein kinase phosphatase-1 (MKP-1) 和增殖，并通過 MKP-1 和 ERK1/2 途徑誘導(dǎo)結(jié)腸癌細(xì)胞凋亡。靶點
| Target | Value |
|
MKP-1
() | |
|
Cdc25A
(Cell-free assay) | 22.3 nM |
|
Cdc25A
(Cell-free assay) | 32 nM(Ki) |
|
Cdc25C
(Cell-free assay) | 40 nM(Ki) |
|
Cdc25B
(Cell-free assay) | 56.9 nM |
體外研究
NSC 95397 (0, 10, and 20 μM; 24 hour) decreases the cell viability of three colon cancer cell lines SW480, SW620, and DLD-1 in a concentration-dependent manner.
NSC 95397(10 μM; 24 hour) upregulates p21 while downregulates CDK4 and CDK6 were d in all three colon cancer cell lines SW480, SW620, and DLD-1 cells.
NSC 95397 (10 μM; 24 hour) reduces the phosphorylation of retinoblastoma protein (Rb) on Ser795 and Ser807/811.
NSC 95397 (20 μM; 24 hours) significantly increases cleaved caspase-9, -3, -7 and PARP levels.
NSC 95397 (10 μM; 6 hours) enhances the phosphorylation of its downstream ERK1/2 at Thr202/Tyr 204.
Cell Viability Assay
| Cell Line: | Human colon cancer cell lines, SW480, SW620, and DLD-1 |
| Concentration: | 0, 10, and 20 μM |
| Incubation Time: | 24 hours |
| Result: | The IC 50 values of NSC 95397 for the cell growth of SW480, SW620, and DLD-1 cells were 9.9, 14.1 and 18.6 μM, respectively. |
Western Blot Analysis
| Cell Line: | SW480, SW620, and DLD-1 cells |
| Concentration: | 10 μM |
| Incubation Time: | 24 hours |
| Result: |
p21 was upregulated while CDK4 and CDK6 were downregulated.
Rduced the phosphorylation of Rb on Ser795 and Ser807/811 |