901-47-3
901-47-3 結(jié)構(gòu)式
基本信息
Nα-Tosyl-L-arginine methyl
Nα-Tosylarginine methyl ester
TOSYL-L-ARGININE METHYL ESTER
Nα-Tosyl-L-arginine methyl ester
Nα-(p-Tolylsulfonyl)-L-arginine methyl ester
Nα-(4-Methylphenylsulfonyl)-L-arginine methyl ester
P-TOLUENESULFONYL-L-ARGININE METHYL ESTER HCL USP/EP/BP
(S)-methyl 5-guanidino-2-(4-methylphenylsulfonamido)pentanoate
5-(diaminomethylideneamino)-2-[(4-methylphenyl)sulfonylamino]pentanoic acid methyl ester
物理化學(xué)性質(zhì)
常見問(wèn)題列表
Anaphase-promoting complex (APC)
The absence of APC substrates, TAME ejects Cdc20 from the APC by promoting Cdc20 auto-ubiquitination in its N-terminal region. Cyclin B1 antagonizes TAME's effect by promoting binding of free Cdc20 to the APC and suppressing Cdc20 auto-ubiquitination.
TAME stabilizes cyclin B1 in
Xenopus
extract by two mechanisms. First, it reduces the k
cat
of the APC
Cdc20
/cyclin B1 complex without affecting the K
m
, slowing the initial ubiquitination of unmodified cyclin B1. Second, as cyclin B1 becomes ubiquitinated, it loses its ability to promote Cdc20 binding to the APC in the presence of TAME. As a result, cyclin B1 ubiquitination terminates before reaching the threshold necessary for proteolysis.