51805-45-9
基本信息
三(2-甲酰乙基)膦鹽酸鹽
三(2-羧乙基)磷鹽酸鹽
3,3',3''-PHOSPHINIDYNE-TRIPROPIONIC ACID HYDROCHLORIDE
TCEP
TCEP HCL
TCEP*HCL
TCEP, HYDROCHLORIDE
TRI(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE
TRIS(2-CARBOXYETHYL)PHOSPHINE, HCL
TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE
TRIS(CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE
TRIS(CARBOXYETHYL)PHOSPHINE &
0.5M TCEP
TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE 99%
TRIS-(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE (TCEP HCL)
Tris(2-carboxyethyl)phosphine-D16DCl
Tris-(2-carboxyethy)phosphine hydrochloride
Tris(2-carboxyethyl)phosphine,hydrochloride,99%
Tris(2-carboxyethyl)phosphonium Hydrochloride
TRIS-(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE (TCEP)
3,3',3''-Phosphinidynetrispropanoic Acid Hydrochloride
物理化學(xué)性質(zhì)
λ: 280 nm Amax: 0.03
安全數(shù)據(jù)
應(yīng)用領(lǐng)域
常見問題列表
三(2-羧基乙基)膦鹽酸鹽(TCEP鹽酸鹽)是一種新型的巰基化DNA的還原劑和去保護(hù)劑。巰基化DNA末端硫原子在溶液中趨向于形成二聚體,特別是在存在氧氣的情況下。這種二聚化大大降低了一些偶聯(lián)反應(yīng)實驗(如DNA在生物感應(yīng)器中的固定)的效率;而在DNA溶液中加入三(2-羧基乙基)膦鹽酸鹽(TCEP鹽酸鹽),反應(yīng)一段時間后除去,就可以降低DNA的二聚化。
TCEP hydrochloride has been introduced which o?ers the prospect of serving as an alternative to the more commonly employed DTT in the NF-κB-DNA binding reactions in vitro, using recombinant p50 protein and a
32
P-labelled κB oligonucleotide. DTT promotes NF-κB-DNA binding in concentrations from 0.25 to 2.6 mM in binding reactions. However, in the presence of 0.25 mM DTT, inhibition of NF-κB binding is seen only at Hg
2+
concentrations greater than 100 μM and results are highly variable. In contrast, TCEP hydrochloride promotes NF-κB-DNA binding in a dose-related manner in concentrations from 0.25 to 6 mM. In the presence of even 6 mM TCEP hydrochloride, Hg
2+
prevents NF-κB-DNA binding at concentrations as low as 20 μM in binding reactions.
The human lactoferrin (hLF) peptide is dissolved in phosphate buffer to a concentration of 0.1 mm. Reduction of the disulfide bonds is obtained by adding a 30-fold molar excess of TCEP hydrochloride with subsequent incubation for 2 h at 37 ℃.
報價日期 | 產(chǎn)品編號 | 產(chǎn)品名稱 | CAS號 | 包裝 | 價格 |
2024/11/11 | XW518054592 | 三(2-羧乙基)膦鹽酸鹽 tris(2-carboxyethyl)phosphine hydrochloride;tcep | 51805-45-9 | 5G | 60元 |
2024/11/08 | H51864 | 三(2-羧乙基)磷鹽酸鹽, 95%, 0.5M 水溶液 Tris(2-carboxyethyl)phosphine hydrochloride, 95%, 0.5M soln. in water | 51805-45-9 | 5ml | 577元 |
2024/11/08 | H51864 | 三(2-羧乙基)磷鹽酸鹽, 95%, 0.5M 水溶液 Tris(2-carboxyethyl)phosphine hydrochloride, 95%, 0.5M soln. in water | 51805-45-9 | 25ml | 2426元 |