35013-72-0
基本信息
(+)生物素-N-琥珀酰亞胺基酯
D-生物素-N-羥基琥珀酰亞胺酯
N-羥基丁二酰亞胺基生物素
N-羥基琥珀酰亞胺基生物素
N-羥基琥珀酰亞胺生物素
(+)-生物素-N-羥基琥珀酰亞胺酯
(+)-生物素-N-羥基琥珀酰亞胺酯
(+)-生物素-N-羥基琥珀酰亞胺酯,98%
BIOTIN (LONG ARM) NHS
BIOTIN-NHS
BIOTIN-N-HYDROXYSUCCINIMIDE
(+)-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
(+)-BIOTIN N-SUCCINIMIDYL ESTER
BIOTIN-OSU
BIOTIN SE
BIOTIN SUCCINIMIDYL ESTER
BIOTINYL-N-HYDROXY-SUCCINIMIDE
(+)-BIOTINYL-N-SUCCINIMIDYL ESTER
BNHS
D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER
D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
D-BIOTIN, SE
D-BIOTIN, SUCCINIMIDYL ESTER
D-BIOTINYL-OSU
NHS-BIOTIN
NHS-D-BIOTIN
物理化學(xué)性質(zhì)
應(yīng)用領(lǐng)域
常見問題列表
NHS生物素試劑可對溶液中的抗體,蛋白質(zhì)和任何其他含伯胺的大分子進行簡單有效的生物素標(biāo)記。 這三種試劑僅在間隔臂長度上有所不同,為研究人員提供了優(yōu)化標(biāo)記和檢測實驗的可能性,其中生物素結(jié)合的空間位阻是重要因素。
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Calculations
:
For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.
1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:
mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.
* 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample
2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction:
mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin
Biotin Labeling Reaction
:
1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations.
2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO.
3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution.
4. Incubate reaction on ice for two hours or at room temperature for 30 minutes.
5. To tests the labeled protein by ELISA or Western blot.