341-88-8
341-88-8 結(jié)構(gòu)式
基本信息
2-N-庚基-4-羥基喹啉N-氧化物
kf8940
C04284
Pyo II
Antibiotic KF-8940
NZPACTGCRWDXCJ-UHFFFAOYSA-N
2-heptyl-4-quinolino1-oxide
2-Heptylquinolin-4-ol 1-oxide
2-Heptyl-4-quinolinol 1-oxide
4-Quinolinol, 2-heptyl-, 1-oxide
物理化學(xué)性質(zhì)
安全數(shù)據(jù)
常見(jiàn)問(wèn)題列表
| Target | Value |
|
Complex III
(Cell-free assay) | 64 nM(Kd) |
|
NDH-2
(Cell-free assay) | 7.3 μM |
HQNO is a potent inhibitor of mitochondrial type II NADH:quinone oxidoreductase (NDH-2) in many species, including
Yarrowia lipolytica
,
S. cerevisiae
,
Gluconobacter oxydans
,
T. gondii
,
P. falciparum
, and
S. aureus
. HQNO targets the Q-site of NDH-2.
HQNO concentrations are varied from 0 to 100?μM and 0 to 300?μM for wild-type (WT) and I379E
C. thermarum
NDH-2 variant, respectively to determine IC
50
values. WT NDH-2 has an IC
50
value of 10.5±1.3?μM HQNO in the presence of 400?μM Menadione (MD). In the presence of 50?μM MD, the IC
50
value for HQNO decreases slightly to 7.3±1.2?μM and near complete inhibition (~15% residual activity) is observed with >50?μM HQNO. At 50?μM MD, HQNO inhibition is observed with an IC
50
value of 54.3±1.2?μM.