2161305-12-8
2161305-12-8 結(jié)構(gòu)式
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IC50: 1.43 μM (Protein tyrosine phosphatase-σ)
DJ001 (5-1000 ng/mL; 3-7 days; BM KSL cells) treatment increases the percentages and numbers of BM KSL cells in culture compared with control cultures. DJ001 treatment also significantly increases the numbers of colony forming cells (CFCs) in 3?day culture of BM KSL cells.
The BM KSL cells are irradiated with 300?cGy and placed in media (containing 20?ng/mL Thrombopoietin, 100?ng/mL stem cell factor (SCF), 50?ng/mL Flt3 ligand, TSF) with and without 1?μg/mL DJ001 for 3 days. DJ001 treatment increases recovery of BM CFCs and multipotent colony-forming unit–granulocyte erythroid monocyte megakaryocyte (CFU-GEMM) colonies compared with control cultures.
Cell Viability Assay
| Cell Line: | BM KSL cells |
| Concentration: | 5 ng/mL, 10 ng/mL, 100 ng/mL, 1000 ng/mL |
| Incubation Time: | 3 days or 7 days |
| Result: | Increased the percentages and numbers of BM KSL cells. Also significantly increased the numbers of colony forming cells (CFCs) in 3?day culture of BM KSL cells. |
DJ001 (5?mg/kg; subcutaneous injection; for 24 hours; female C57BL/6 mice) significantly decreases the percentage of apoptotic BM KSL cells in C57BL/6 mice at 24?h following 500?cGy TBI. DJ001 suppresses radiation-induced HSC apoptosis via activation of the RhoGTPase, RAC1, and induction of BCL-X L .
| Animal Model: | Female C57BL/6 mice (8-10-week-old) irradiated with total body irradiation (TBI) |
| Dosage: | 5?mg/kg |
| Administration: | Subcutaneous injection; for 24 hours |
| Result: | Significantly decreased the percentage of apoptotic BM KSL cells in C57BL/6 mice at 24?h following 500?cGy TBI. |