1404-90-6
1404-90-6 結(jié)構(gòu)式
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一種萬古霉素菌株及其發(fā)酵產(chǎn)萬古霉素的方法，包括以下步驟：
1）將萬古霉素生產(chǎn)菌株進(jìn)行液體發(fā)酵培養(yǎng)基培養(yǎng)，液體發(fā)酵種子培養(yǎng)基 組成為酵母粉3～6g/L，麥芽提取物3～5g/L，蛋白胨10～15g/L，葡萄糖 15～20g/L，pH為6.8；種子培養(yǎng)條件為：28～30℃，裝液量40/250mL， 搖床轉(zhuǎn)速150～220rpm，培養(yǎng)24～48h；液體發(fā)酵培養(yǎng)基組成為糊精100～150g/L，土豆蛋白15～25g/L，黃豆粉15～25g/L，磷酸氫二鉀0.1～0.5g/L，消沫劑1～3g/L，氯化鈉1.2～1.6g/L，pH 6.8～7.2，培養(yǎng)條件為28～30℃，裝液量40/250mL，搖床轉(zhuǎn)速150～220rpm，培養(yǎng)90～130h，所得溶液為 含凈抗生素活性洗脫液；
2）在沉淀容器中沉淀步驟1）中所得的含凈抗生素活性洗脫液，得到晶體漿料，并且攪拌該晶體漿料，將PH值連續(xù)調(diào)節(jié)至所需程度，直至完全沉淀，得到晶體沉淀物；
3）將步驟2）得到的晶體沉淀物溶解為水溶液，然后通過陰離子交換樹脂將其轉(zhuǎn)為萬古霉素。
Vancomycin is a large glycopeptide compound with a molecular weight of 1450 Da. Vancomycin is a unique glycopeptide structurally unrelated to any currently available antibiotic. It also has a unique mode of action inhibiting the second stage of cell wall synthesis of susceptible bacteria. Vancomycin is active against a large number of species of Gram-positive bacteria, such as Staphylococcus aureus, Staph. epidermidis, Str. agalactiae, Str. bovis, Str. mutans, viridans streptococci, enterococci .
Vancomycin is administered intravenously, with a standard infusion time of at least 1 h, to minimize infusion-related adverse effects. Subjects with normal creatinine clearance, vancomycin has an α-distribution phase of 30 min to 1 h and a β-elimination half-life of 6-12 h. The volume of distribution is 0.4–1 L/kg. The binding of vancomycin to protein ranges from 10% to 50%. Factors that affect the overall activity of vancomycin include its tissue distribution, inoculum size, and protein-binding effects. Vancomycin treatment of infected mice is associated with improved clinical, diarrhea, and histopathology scores and survival during treatment.