109944-15-2
中文名稱
幾夫堿
英文名稱
KIFUNENSINE
CAS
109944-15-2
分子式
C8H12N2O6
分子量
232.19
MOL 文件
109944-15-2.mol
更新日期
2023/03/20 15:41:23
109944-15-2 結(jié)構(gòu)式
基本信息
中文別名
幾夫堿 英文別名
FR 900494KIFUNENSINE
Kifunensine min. 99%
[5R-(5α,6β,7α,8α,8aα)]-
KIFUNENSINE, KITASATOSPORIA KIFUNENSE
Hexahydro-6R,7S,8aS-trihydroxy-5R-(hydroxymethyl)-imidazo[1,2-a] pyridine-2,3-dione
(5R,6R,7S,8R,8AS)-6,7,8-trihydroxy-5-(hydroxymethyl)hexahydroimidazo[1,2-a]pyridine-2,3-dione
(5R,6R,7S,8R,8aS)-Hexahydro-6,7,8-trihydroxy-5-(hydroxymethyl)-imidazo[1,2-a]pyridine-2,3-dione
FR 900494, Hexahydro-6R,7S,8aS-trihydroxy-5R-(hydroxymethyl)-imidazo[1,2-a] pyridine-2,3-dione
Imidazo1,2-apyridine-2,3-dione, hexahydro-6,7,8-trihydroxy-5-(hydroxymethyl)-, (5R,6R,7S,8R,8aS)-
物理化學(xué)性質(zhì)
熔點>280°C
密度1.85±0.1 g/cm3(Predicted)
儲存條件−20°C
溶解度二次蒸餾水中的溶解度：50MM
酸度系數(shù)(pKa)9.61±0.70(Predicted)
形態(tài)白色固體
顏色白色
生物來源synthetic (organic)
水溶解性Soluble to 5 mM in water with sonication
穩(wěn)定性干燥并儲存在-20°C
常見問題列表
生物活性
Kifunensine 是可從放線菌中分離得到的 I 類 α-mannosidases 有效選擇性抑制劑，可阻斷 α-mannosidases I 修剪糖蛋白上的甘露糖殘基。Kifunensine 可抑制內(nèi)質(zhì)網(wǎng)相關(guān)蛋白降解途徑。體外研究
Kifunensine, an alkaloid from actinomycete Kitasatosporia kifunense 9482, is the most efficient nhibitor of a-mannosidase I, blocking N-glycan synthesis at an8GlcNAc 2 (Man8) or Man9GlcNAc 2 (Man9) stage.
RT-PCR.
| Cell Line: | Hybridoma cells expressing a human IgG1 monoclonal Antibody [3] . |
| Concentration: | 2 μg/mL. |
| Incubation Time: | 4 days. |
| Result: |
Significantly reduced the lentil lectin binding.
Kifunensine is the most effective among the inhibitors tested in producing antibodies containing oligomannose residues without fucose. |
體內(nèi)研究
| Animal Model: | BALB/c mice. |
| Dosage: | 5 mg/kg. |
| Administration: | IV via tail vein. |
| Result: | There was no significant difference in the serum levels of anti-TEM B mAb generated from cells treated with or without kifunensine in the 7-day period. |