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ChemicalBook--->CAS DataBase List--->81-93-6

81-93-6

81-93-6 Structure

81-93-6 Structure
IdentificationBack Directory
[Name]

CI 50200
[CAS]

81-93-6
[Synonyms]

nsc9855
CI 50200
CI NO 50200
schultzno.958
PHENOSAFRANIN
PHENOSAFRANINE
Phenosafeanine
phenosofranine
phenosofrinine
safrininbextra
Safranine B Extra
phenosafranine,chloride
PHENOSAFRANINE FOR MICROSCOPY
3,7-diamino-5-phenyl-phenaziniuchloride
3,7-DIAMINO-5-PHENYLPHENAZINIUM CHLORIDE
Phenazinium,3,7-diamino-5-phenyl-,chloride
2,8-DiaMino-10-phenyl-phenaziniuM Chloride
3,7-Diamino-5-phenylphenazin-5-ium chloride
[EINECS(EC#)]

201-387-0
[Molecular Formula]

C18H15ClN4
[MDL Number]

MFCD00036335
[MOL File]

81-93-6.mol
[Molecular Weight]

322.79
Chemical PropertiesBack Directory
[Appearance]

green crystalline powder
[Melting point ]

300 °C
[Boiling point ]

478.49°C (rough estimate)
[density ]

1.1738 (rough estimate)
[refractive index ]

1.6110 (estimate)
[storage temp. ]

room temp
[Colour Index ]

50200
[form ]

Solid
[color ]

Green to dark green
[λmax]

519 nm
[Merck ]

13,7333
[BRN ]

3642082
[CAS DataBase Reference]

81-93-6
Hazard InformationBack Directory
[Chemical Properties]

green crystalline powder
[Uses]

A bacterial stain used in microscopy.
[Definition]

ChEBI: An organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms.
[Uses]

Biological stain.
[Biochem/physiol Actions]

Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally.
[Staining Procedures]

Phenosafranin is used to distinguish living from dead cells. A 0.1 % aqueous solution, it is taken up by dead or abnormal cells immediately, while living cells remain unstained for at least 30 min.
[Purification Methods]

Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.]
Safety DataBack Directory
[Hazard Codes ]

Xi
[Risk Statements ]

36/37/38
[Safety Statements ]

26-36
[WGK Germany ]

3
[RTECS ]

SG1630000
Spectrum DetailBack Directory
[Spectrum Detail]

Phenosafranin(81-93-6)MS
Phenosafranin(81-93-6)IR1
Phenosafranin(81-93-6)IR2
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