| Identification | More | [Name]
Janus Green B | [CAS]
2869-83-2 | [Synonyms]
3-DIETHYLAMINO-7-(4-DIMETHYLAMINOPHENYLAZO)-5-PHENYLPHENAZINIUM CHLORIDE
ACIDUM BORICUM
BORACIC ACID
BORIC ACID
BORIC ACID ABSORBANT
Boron trihydroxide
CI 11050
CI NO 11050
DIAZINE GREEN
DIAZIN GREEN S
JANUS GREEN
JANUS GREEN B
KJELDHAL REAGENTS BORIC ACID
KJEL-SORB
OPTIBOR(TM)
ORTHOBORIC ACID
UNION GREEN B
3-(diethylamino)-7-((p-(dimethylamino)phenyl)azo)-5-phenyl-phenaziniuchlor
3-(diethylamino)-7-((p-(dimethylamino)phenyl)azo)-5-phenylphenaziniumchlorid
3-(diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenyl-phenaziniuchlor | [EINECS(EC#)]
220-695-6 | [Molecular Formula]
BH3O3 | [MDL Number]
MFCD00011337 | [Molecular Weight]
61.83 | [MOL File]
2869-83-2.mol |
| Chemical Properties | Back Directory | [Appearance]
green powder | [Melting point ]
160 °C (dec.)(lit.)
| [vapor pressure ]
2.6 mm Hg ( 20 °C)
| [storage temp. ]
2-8°C | [solubility ]
H2O: 1 M hot, clear, colorless
| [Colour Index ]
11050 | [form ]
Powder | [color ]
≤10(APHA)
| [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
Soluble in water (20 mg/ml at 20°C), ethanol, and slightly soluble in alcohol. | [ε(extinction coefficient)]
≥28000 at 651-677nm in ethanol: water (1:1) at 0.01g/L | [λmax]
660nm, 395nm | [Merck ]
14,5255 | [Biological Applications]
Antimalarialagents; diagnosis of diseases related to amyloid accumulation; diagnostic assays; detecting fungi; nucleic acids; sugars | [InChIKey]
XXACTDWGHQXLGW-UHFFFAOYSA-M | [CAS DataBase Reference]
2869-83-2(CAS DataBase Reference) | [EPA Substance Registry System]
2869-83-2(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
T,Xn | [Risk Statements ]
R60:May impair fertility.
R40:Limited evidence of a carcinogenic effect. | [Safety Statements ]
S22:Do not breathe dust .
S24/25:Avoid contact with skin and eyes .
S36:Wear suitable protective clothing . | [WGK Germany ]
3
| [RTECS ]
SG1633000
| [TSCA ]
Yes | [HS Code ]
32041900 | [Safety Profile]
Poison by
intraperitoneal and intravenous routes.
Moderately toxic by ingestion, skin contact,
subcutaneous, and intramuscular routes.
Human systemic effects by inhalation:
nausea or vomiting. A skin and severe eye
irritant. Flammable liquid when exposed to
heat or flame; can react with oxidizing
materials. To fight fire, use alcohol foam,
CO2, dry chemical. When heated to
decomposition it emits toxic fumes of NOx.
See also AMINES. |
| Hazard Information | Back Directory | [Chemical Properties]
green powder | [Uses]
As dye for cotton, wool; as biological stain; in electrodeposition of copper, Brown, Fellows, US 2882209 (1959 to Udylite Res. Corp.). | [Definition]
ChEBI: Janus Green B chloride is an organic chloride salt. It has a role as a dye. It contains a Janus Green B cation. | [Biological Activity]
Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thusthis dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomoleculesnucleic acids and chromosomes. In additionit is also used to stain various living organisms including fungiyeast cellbrainspinal cordsperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation. | [Purification Methods]
The dye dissolves in H2O to give a bluish violet solution which becomes colourless when made 10M in NaOH. It dissolves in EtOH to give a blue-violet colour, filter from insoluble material, then add dry Et2O whereby the dye separates out leaving a small amount of blue colour in solution. Filter off the solid and dry it in a vacuum. Store it in a dark bottle. [Colour Index Vol 4, 3rd edn, 4015 1971.] |
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