| Identification | Back Directory | [Name]
EGS | [CAS]
70539-42-3 | [Synonyms]
EGS
EGNHS
HI-EFF-2B
LAC-4R-886
EGS Crosslinker
ethyleneglycolylbis(succinimidylsuccinate
Ethlene glycol bis(succinimidyl succinate)
ETHYLENE GLYCOL BIS(SUCCINIMIDYL SUCCINATE)
DI(N-SUCCINIMIDYL) ETHYLENE GLYCOL DISUCCINATE
Ethylene glycol bis(succiniMidylsuccinate)(EGS)
ETHYLENE GLYCOL DISUCCINATE DI(N-SUCCINIMIDYL) ESTER
ETHYLENE GLYCOL BIS-(SUCCINIC ACID*N-HYD ROXYSUCCINI
ethylene glycol-bis(succinic acid n-hydroxysuccinimide
ETHYLENE GLYCOL-BIS(SUCCINIC ACID N-HYDROXYSUCCINIMIDE ESTER)
EGS Crosslinker (ethylene glycol bis(succinimidyl succinate))
Bis(2,5-dioxopyrrolidin-1-yl) O,O'-ethane-1,2-diyl disuccinate
Ethylene glycol bis(succininic acid) N-hydroxysucciniMide ester
ethane-1,2-diyl bis{4-[(2,5-dioxopyrrolidin-1-yl)oxy]-4-oxob...
Ethylene Glycol Bis(succinic Acid N-Hydrosuccinimide Ester)
Ethyleneglycol-O,O''-bis-(succinic acid N-hydroxysuccinimide ester)
ethane-1,2-diyl bis{4-[(2,5-dioxopyrrolidin-1-yl)oxy]-4-oxobutanoate}
Butanoic acid, 4-[(2,5-dioxo-1-pyrrolidinyl)oxy]-4-oxo-, 1,2-ethanediyl ester
Butanedioic acid, 1,1'-(1,2-ethanediyl) 4,4'-bis(2,5-dioxo-1-pyrrolidinyl) ester
4-O-(2,5-dioxopyrrolidin-1-yl) 1-O-[2-[4-(2,5-dioxopyrrolidin-1-yl)oxy-4-oxobutanoyl]oxyethyl] butanedioate
Di(N-succinimidyl) ethylene glycol disuccinate, EGS, Ethylene glycol bis(succinimidyl succinate), Ethylene glycol disuccinate di(N-succinimidyl) ester | [Molecular Formula]
C18H20N2O12 | [MDL Number]
MFCD00049057 | [MOL File]
70539-42-3.mol | [Molecular Weight]
456.36 |
| Chemical Properties | Back Directory | [Boiling point ]
603.4±65.0 °C(Predicted) | [density ]
1.52±0.1 g/cm3(Predicted) | [storage temp. ]
2-8°C | [solubility ]
acetic acid: water (1:1): ≤50mg/mL | [form ]
powder | [color ]
White to off-white | [BRN ]
9167119 | [InChI]
InChI=1S/C18H20N2O12/c21-11-1-2-12(22)19(11)31-17(27)7-5-15(25)29-9-10-30-16(26)6-8-18(28)32-20-13(23)3-4-14(20)24/h1-10H2 | [InChIKey]
QLHLYJHNOCILIT-UHFFFAOYSA-N | [SMILES]
O(N1C(CCC1=O)=O)C(=O)CCC(=O)OCCOC(=O)CCC(=O)ON1C(CCC1=O)=O |
| Hazard Information | Back Directory | [Description]
EGS Crosslinker(70539-42-3) is a cleavable crosslinker which can be cleaved using hydroxyamine. EGS Crosslinkers contain two NHS ester groups which can react with primary amines.
| [Uses]
bromoacetate amine and sulfhydryl reactive homobifunctional cross-linking reagent | [Uses]
Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) has been used as a cross-linking agent. | [Biochem/physiol Actions]
Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is a homobifunctional, cleavable cross-linking reagent. It is mainly coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). The linkage provides a 12-atom spacer. | [Properties and Applications]
EGS crosslinker, Ethylene glycolbis(succinimidyl succinate), is a water-insoluble homobifunctional crosslinker that must be dissolved in DMSO or DMF before addition to an aqueous reaction. EGS crosslinking takes place through amine-reactive NHS esters at both ends of a 16.1-angstrom spacer arm. The 12-atom spacer arm of the EGS crosslinking reagent is cleavable and the spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule and can be used to label intracellular proteins since the molecule is cell membrane permeable. EGS crosslinker is often used in ChIP assays to determine DNA-protein binding interactions. The directly water-soluble analog of EGS is Sulfo-EGS.
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