| Identification | More | [Name]
Fluorescamine | [CAS]
38183-12-9 | [Synonyms]
4'-PHENYL-SPIRO[2-BENZOFURAN-1(3H),2'(3'H)-FURAN]-2',3-DIONE
4-PHENYL SPIRO-[FURAN-2(3H),1-PHTHALAN]-3,3'-DIONE
4-PHENYLSPIRO[FURAN-2(3H),1'-PHTHALAN]-3,3'-DIONE
FLUORESCAMINE
FLUORESCAMINE SPRAY REAGENT
FLURAM
FLURAM(R)
RO 20-7234
SPIRO[FURAN-2(3H),1'(3'H)-ISOBENZOFURAN]-3,3'-DIONE, 4-PHENYL-
4-phenylspiro[furan-2(3H),1'(3'H)-isobenzofuran]-3,3'-dione
FLURAM(R) (FLUORESCAMINE), FOR FLUORESCE NCE
FluorescamineA.R.
Fluorescamine, pure
Fluorescamine [for HPLC Labeling]
FLUORESCAMINE extrapure AR
4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3μ-dione, Fluorescamine
Supelco(R) TLC Spray Reagents | [EINECS(EC#)]
253-814-5 | [Molecular Formula]
C17H10O4 | [MDL Number]
MFCD00005928 | [Molecular Weight]
278.26 | [MOL File]
38183-12-9.mol |
| Chemical Properties | Back Directory | [Appearance]
white powder | [Melting point ]
153-157 °C (lit.) | [Boiling point ]
381.08°C (rough estimate) | [density ]
1.2661 (rough estimate) | [refractive index ]
1.5447 (estimate) | [Fp ]
-17 °C | [storage temp. ]
2-8°C
| [solubility ]
acetonitrile: soluble
| [form ]
powder
| [color ]
off-white to yellow
| [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
Soluble in acetone, ethanol, chloroform, dimethylsulfoxide and acetonitrile. Slightly soluble in water. | [Merck ]
14,4158 | [BRN ]
921143 | [CAS DataBase Reference]
38183-12-9(CAS DataBase Reference) | [NIST Chemistry Reference]
|
| Safety Data | Back Directory | [Hazard Codes ]
F,Xi | [Risk Statements ]
R11:Highly Flammable.
R36/38:Irritating to eyes and skin .
R66:Repeated exposure may cause skin dryness or cracking.
R67:Vapors may cause drowsiness and dizziness.
R36:Irritating to the eyes.
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S22:Do not breathe dust .
S24/25:Avoid contact with skin and eyes .
S36/37:Wear suitable protective clothing and gloves .
S33:Take precautionary measures against static discharges .
S29:Do not empty into drains .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S16:Keep away from sources of ignition-No smoking .
S9:Keep container in a well-ventilated place .
S37/39:Wear suitable gloves and eye/face protection . | [RIDADR ]
UN 1090 3/PG 2
| [WGK Germany ]
3
| [F ]
10-21 | [HS Code ]
29322090 |
| Hazard Information | Back Directory | [Chemical Properties]
white powder | [Uses]
Analytical reagent. | [Uses]
Fluorescamine is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Reaction takes place under mild conditions. Low background due to hydrolysis.
| [Uses]
For fluorescent detection of amino acids, peptides and proteins.Fluorescamine plays an important role as a reagent for the detection of amines, peptides and proteins. It is also used as a substrate for measuring protease activity. It is utilized in the detection and quantitation of residual aminopenicillins and sulfonamides in honey by HPLC. It finds application in the determination of lisinopril in human plasma and urine. | [Synthesis Reference(s)]
Journal of the American Chemical Society, 94, p. 5927, 1972 DOI: 10.1021/ja00771a084 | [Purification Methods]
Fluram is a non-fluorescent reagent that reacts with primary amines to form highly fluorescent compounds. Purify it by dissolving (~1g) in Et2O/*C6H6 (1:1, 180 mL), washing with 1% aqueous NaHCO3 (50mL), drying (Na2SO4), and evaporating in a vacuum. Dissolve the residue in warm CH2Cl2 (5mL), dilute with Et2O (12mL) and refrigerate. Collect the solid and dry it in a vacuum. IR (CHCl3): 1810, 1745, 1722, 1625 and 1600 cm-1, and 1HNMR (CDCl3): 8.71 (s, -OHC=). [Weigele et max al. J Am Chem Soc 94 5927 1972, Weigele et al. J Org Chem 41 388 1976, Lai Methods Enzymol 47 236 1977.] Forskolin (Colforsin, Coleonol, 5-[acetyloxy]-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7, 10a-penta-methyl-[3R -{3 |
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