| Identification | Back Directory | [Name]
KISSPEPTIN-10 | [CAS]
374675-21-5 | [Synonyms]
KiSS-1
Kisspetin-10
KISSPEPTIN-10
KISS-1 (112-121)
H-YNWNSFGLRF-NH2
YNWNSFGLRF - NH2
METASTIN (45-54)
Kisspeptin-10 acetate
Kisspeptin 10 (human)
Kisspeptin 4-13 acetate
METASTIN (HUMAN, 45-54)
METASTIN (45-54), HUMAN
KISS-1 (112-121) (HUMAN)
KISSPEPTIN-13 (4-13) (HUMAN)
Kisspeptin-14 (5-14) (human)
Kisspeptin-54 (45-54) (human)
METASTIN (45-54) AMIDE, HUMAN
GENE PRODUCT (112-121) AMIDE, HUMAN
Kisspeptin-10 Metastin (45-54), Human
Kisspeptin-10 (Kp-10), Metastin (45-54)
KISS-1 GENE PRODUCT (HUMAN, 112-121 AMIDE)
TYR-ASN-TRP-ASN-SER-PHE-GLY-LEU-ARG-PHE-NH2
H-TYR-ASN-TRP-ASN-SER-PHE-GLY-LEU-ARG-PHE-NH2
KISS-1TYR-ASN-TRP-ASN-SER-PHE-GLY-LEU-ARG-PHE-NH2
KISS-1 GENE PRODUCT (HUMAN, 122-121 AMIDE), KISSPEPTIN-10
Gene product (112-121) amide, human, KiSS-1, Kisspeptin-10
MALIGNANT MELANOMA METASTASIS-SUPPRESSOR KISS-1 (112-121) (HUMAN)
L-Phenylalaninamide, L-tyrosyl-L-asparaginyl-L-tryptophyl-L-asparaginyl-L-seryl-L-phenylalanylglycyl-L-leucyl-L-arginyl-
Malignant MelanoMa Metastasis-Suppressor KiSS-1 (112-121) (huMan), Metastin (45-54) (huMan), KiSS-1 (112-121) (huMan), Kisspeptin-14 (5-14) (huMan), Kisspeptin-54 (45-54) (huMan), Kisspentin-10 | [EINECS(EC#)]
675-121-5 | [Molecular Formula]
C63H83N17O14 | [MDL Number]
MFCD03452696 | [MOL File]
374675-21-5.mol | [Molecular Weight]
1302.44 |
| Chemical Properties | Back Directory | [density ]
1.46±0.1 g/cm3(Predicted) | [storage temp. ]
-20°C | [solubility ]
DMF: 15 mg/ml; DMSO: 15 mg/ml; DMSO:PBS(pH 7.2) (1:2): 0.33 mg/ml | [form ]
A crystalline solid | [pka]
9.96±0.15(Predicted) | [color ]
White to off-white | [Water Solubility ]
Soluble in water at 1mg/ml | [Sequence]
H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2 | [InChIKey]
RITKWYDZSSQNJI-INXYWQKQSA-N |
| Hazard Information | Back Directory | [Description]
Kisspeptin 10 (human)(374675-21-5) is a 10 amino acid peptide that corresponds to the bioactive C-terminal 45-54 amino acids of metastin, a metastasis suppressor gene product. It acts as a potent agonist of GPR54 (OT7T175, AXOR12), with approximately 8-fold higher binding affinity than metastin (Ki values of 0.042 and 0.34 nM, respectively, for displacement of metastin (40-54)). Metastin (45-54) induces calcium mobilization in GPR54-transfected cells (EC50 = 0.18-1.1 nM). It inhibits the migration of GPR54-transfected CHO cells at concentrations of 10-100 nM, equaling the potency of full length metastin. | [Uses]
Metastin (45-54) is a potent and selective agonist of AXOR12 and hOT7T175. | [Biological Functions]
Metastin serves as a tumor suppressor by hindering the growth of secondary tumors. When KISS1R (KISS1 receptor) is activated upon binding with KISS1, it boosts intracellular calcium levels and activates MAPKs, which in turn limit cell mobility and proliferation. Metastin is a potential therapeutic target for various cancers, including melanoma, thyroid, bladder, squamous cell carcinoma of the esophagus, gastric, hepatocellular, and breast cancers.
In addition to its role in cancer suppression, metastin plays a critical role in reproduction. It regulates the hypothalamic-pituitary-gonadal axis by modulating gonadotropin-releasing hormone (GnRH) secretion, which influences the onset of puberty. Gene mutations can lead to central precocious puberty (CPP) in males, while impaired metastin signaling can result in isolated hypogonadotropic hypogonadism (IHH) in both humans and mice. Metastin can also elevate plasma levels of gonadotropins (follicle-stimulating hormone and luteinizing hormone) and promote ovulation in prepubescent female rats. | [General Description]
Metastin is also known as kisspeptin 1 (KISS1). It is encoded by KISS1 gene, that is mapped to human chromosome 1q32. Metastin is a tumor suppressor protein, made up of 145 amino acids. The protein fragments contain RFamide motif at the C- terminal end. The encoded protein is expressed in the human hypothalamus, gonads, placenta, liver and pancreas. This novel?peptide?is mainly isolated from the human placenta. | [Biological Activity]
The study revealed that the down regulation of galactose and up regulation of glycogenic amino acids (GAAs), including L-alanine, L-glutamic acid, L-methionine, L-proline and L-valine, in the kisspeptin-10 group suggests the accelerated carbohydrate metabolism, which indicates a negative energy balance. Moreover, in the kisspeptin-10-treated rats, high levels of GAAs that were possibly derived from protein decomposition might cause a negative nitrogen balance. It was found that increases of ketogenic amino acids such as tyrosine indicate a disturbance in glucose use, which might be induced by kisspeptin-10 in this study. In contrast, cholesterol increased significantly in the rats treated with kisspeptin-10, suggesting disrupted lipid metabolism, which might result from a lipolysis blockade[1].
| [Biochem/physiol Actions]
Metastin functions as a tumor suppressor via inhibiting secondary tumor growth. Activation of KISS1R (KISS1 receptor), upon binding KISS1, enhances intracellular calcium and activates MAPKs, which restricts cell motility and proliferation. Metastin acts as a potential therapeutic target for various cancers including melanoma, thyroid cancer and bladder cancer, squamous cell carcinoma of the esophagus gastric cancer, hepatocellular carcinoma and breast cancer. Metastin has a crucial role in reproduction. It regulates hypothalamic-pituitary-gonadal axis role via controlling gonadotropin-releasing hormone (GnRH) secretion and determines the onset of puberty. Mutation of the gene leads to central precocious puberty (CPP) in male and dysfunction of metastin signaling causes isolated hypogonadotropic hypogonadism (IHH) in humans and mice. Metastin has the ability to enhance the?plasma levels?of?gonadotropins (follicle-stimulating hormone and luteinizing hormone) and stimulate ovulation?in prepubertal female rats. | [Mechanism of action]
The kisspeptin (Kp) precursor contains 145 amino acids and can be hydrolyzed into Kp-54, Kp-14, Kp-13 and Kp-10. Kp-10, the shortest subtype with high activity, plays a vital role in many tissues. Kisspeptin 10 inhibited the expression of vascular endothelial growth factor (VEGF) in human umbilical vein endothelial cells, the main inducer of high vascular permeability (VP). Moreover, Kp-10 also promotes follicle maturation, ovulation and progesterone production. Exogenous Kp-10 injection decreased VP and VEGF by enhancing Kiss1r in OHSS rats without affecting ovulation [3]. | [storage]
Store at -20°C | [Clinical claims and research]
Kisspeptin-10 level is useful in assessing the severity of preeclampsia and can be a novel marker downregulated in pregnant women with preeclampsia, especially in those who also developed impaired uteroplacental perfusion or intrauterine growth restriction[2]. | [References]
[1] Zhang Y, et al. The effects of kisspeptin-10 on serum metabolism and myocardium in rats. PLOS ONE, 2017; 12: e0179164.
[2] Ziyaraa M, et al. Correlation of Kisspeptin-10 level and fetal well-being in preeclamptic patients. Taiwanese Journal of Obstetrics and Gynecology, 2016; 55: 840-846.
[3] Zhai J, et al. Kisspeptin-10 inhibits OHSS by suppressing VEGF secretion. Reproduction, 2017; 154: 355–362.
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