| Identification | Back Directory | [Name]
SULFORHODAMINE B | [CAS]
3520-42-1 | [Synonyms]
red106
AKA106
ACID RED
CI 45100
redno.106
foodred106
XYLENE RED
KITON RED S
ACID RED XB
ACID RED 52
CI NO 45100
ACIDREDNO52
Acid Rose B
KITTON RED S
XYLENE RED B
AcidroseredB
C.I.Acidred52
fenazopinkxxb
Dyacid Red 4B
Acid Red 52 0
Projet Red OAM
Conacid Red EB
Acid Rhodamine
solarrhodamineb
FOOD RED NO 106
ERIO ACID RED XB
ACID RHODAMINE B
amacidrhodamineb
acidleatherredkb
SULFORHODAMINE B
PHLOXINRHODAMINE
AMIDO RHODAMINE B
KITON RHODAMINE B
Lissamine rhodamin
PHLOXINE RHODAMINE
aizenfoodredno.106
KitonRedSC.I.14895
Dinacid Rhodamine B
Indacid Rhodamine B
San-Ei Gen Acid Red
Triacid Rhodamine B
LISSAMINE RHODAMINE
Nylosan Rhodamine B
Kayacyl Rhodamine FB
Dycosacid Rhodamine B
Vibracolor Red ARE52.
Hodgsons Rhodamine FB
LISSAMINE RHODAMINE B
pontacylbrilliantpink
Daiwa Acid Rhodamine B
Sandolan Rhodamine E-B
Aizen Acid Rhodamine BG
brilliantacidrhodamineb
Acid red 52 (C.I. 45100)
Colocid Rhodamine B,BH,BN
LISSAMINE RHODAMINE B-200
Duasyn Acid Rhodamine B 01
brilliantsuperlanrhodamineb
brilliantsuperlanrhodamine2b
sulforhodamine b sodium salt
SULFORHODAMINE B (C.I. 45100)
ADC Brilliant Sulpho Rhodamine B
SULFORHODAMINE B MONOSODIUM SALT
sulforhodamine B cell culture tested
Acid Red
Sulforhodamine B
Xylene Red
LISSAMINE RHODAMINE B-200 ON CELITE 5%
Acid Red 52, Sulforhodamine B monosodium salt
SULFORHODAMINE B MONOSODIUM SALT, FOR FL UORESCENCE
Xylene red B
Acid Red 52
Sulforhodamine B monosodium salt
SULFORHODAMINE B CELL CULTURE REAGENT DYE CONTENT APPOX. 75%
(6-(diethylamino)-9-(2,4-disulfophenyl)-3h-xanthen-3-ylidene)diethyl-ammoniu
hydrogen 3,6-bis(diethylamino)-9-(2,4-disulphonatophenyl)xanthylium, sodium salt
2-(6-Diethylamino-3-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate
Xanthylium, 3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-, inner salt, sodium salt
XanthyliuM,3,6-bis(diethylaMino)-9-(2,4-disulfophenyl)-, inner salt, sodiuM salt (1:1)
Xanthylium, 3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-, hydroxide, inner salt, sodium salt | [EINECS(EC#)]
222-529-8 | [Molecular Formula]
C27H29N2NaO7S2 | [MDL Number]
MFCD00010180 | [MOL File]
3520-42-1.mol | [Molecular Weight]
580.65 |
| Chemical Properties | Back Directory | [Appearance]
Deep red powder | [density ]
1.472[at 20℃] | [storage temp. ]
room temp | [solubility ]
methanol: 1 mg/mL
| [Colour Index ]
45100 | [form ]
powder
| [color ]
Brown to Very Dark Purple | [biological source]
synthetic (organic) | [Water Solubility ]
95.3g/L at 20℃ | [ε(extinction coefficient)]
≥85000 at 554-560nm in 0.1 M NaOH in methanol at 0.005g/L | [λmax]
554 nm | [BRN ]
3886008 | [InChIKey]
XCRMYPBRGZRYEU-UHFFFAOYSA-N | [SMILES]
S(C1C=C(S(O)(=O)=O)C=CC=1C1C2=CC=C(N(CC)CC)C=C2[O+]=C2C=C(N(CC)CC)C=CC=12)([O-])(=O)=O.[NaH] | [LogP]
-2.2 at 20℃ | [EPA Substance Registry System]
C.I. Acid Red 52 (3520-42-1) |
| Hazard Information | Back Directory | [Chemical Properties]
Deep red powder | [Application]
Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material. | [Uses]
Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material. | [Uses]
Sulforhodamine B sodium salt has been used:
- for relative total cell mass quantification
- to investigate the viscosity of coacervates
- as a molecular rotor for microviscosity determination in aerosol droplets
| [Uses]
Sulforhodamine B Sodium Salt is a rhodamine dye widely used in nonpermanent tattoos. Studies show that Sulforhodamine B can be used as a potential new therapeutic agent for the treatment of the acute respiratory distress syndrome. Dyes and metabolites, Environmental Testing | [Definition]
ChEBI: An organic sodium salt having 4-[3,6-bis(diethylamino)-2,7-dimethylxanthenium-9-yl]benzene-1,3-disulfonate as the counterion. | [Preparation]
4-Formylbenzene-1,3-disulfonic acid and 3-(Diethylamino)phenol condensation, the products with sulfuric acid dehydration, and ferric chloride oxidation, and finally into sodium salt. | [General Description]
Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination. | [Flammability and Explosibility]
Notclassified | [Biochem/physiol Actions]
Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening. | [Properties and Applications]
bright blue light pink. Dark red light brown powder. Soluble in water, in blue light red, with fluorescence, also soluble in alcohol. The strong sulfuric acid dyes is orange, diluted for red, with sodium hydroxide solution is blue light red. Mainly used for wool, silk and polyamide fiber dyeing, also can be used for wool and a variety of fabric dyeing, still can be in wool and silk fabric printing directly, also can used for leather dyeing.
|
Standard
|
Light Fastness
|
Soaping
|
Persperation Fastness
|
Oxygen bleaching
|
Fastness to seawater
|
|
Fading
|
Stain
|
Fading
|
Stain
|
Fading
|
Stain
|
|
ISO
|
3
|
3-4
|
3
|
4
|
1
|
2
|
3
|
2
|
|
AATCC
|
2-3
|
3
|
3
|
3
|
1
|
2
|
4
|
2-3
|
|
|
|