| Identification | More | [Name]
5-Aminofluorescein | [CAS]
3326-34-9 | [Synonyms]
1-AMINOFLUORESCEIN
1-FLUORESCEINAMINE
4-AMINOFLUORESCEIN
4-AMINOFLUORESCEIN ISOMER I
5-amino-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid
5-AMINO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE
5-AMINOFLUORESCEIN
5-AMINOFLUORESCEIN ISOMER I
AMINOFLUORESCEIN
FLUORESCEINAMINE
FLUORESCEINAMINE, ISOMER 1
FLUORESCEINAMINE ISOMER I
9’-[9h]xanthen]-3-one,5-amino-3’,6’-dihydroxy-spiro[isobenzofuran-1(3h
Fluoresceinamine，isomerⅡ
5-AMINOFLUORESCEIN, FOR FLUORESCENCE
FLUORESCEIN AMINE ISOMER I 99%
4-AMINOFLUORESCEIN (ISOMER 1)
Fluoresceinamine isomer I, pure
FLUORESCENAMINEISOMERI
6-Amino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one | [EINECS(EC#)]
222-043-6 | [Molecular Formula]
C20H13NO5 | [MDL Number]
MFCD00005052 | [Molecular Weight]
347.32 | [MOL File]
3326-34-9.mol |
| Chemical Properties | Back Directory | [Appearance]
Dark Red Solid | [Melting point ]
223 °C (dec.)(lit.) | [Boiling point ]
481.89°C (rough estimate) | [density ]
1.3724 (rough estimate) | [refractive index ]
1.5180 (estimate) | [storage temp. ]
2-8°C
| [solubility ]
DMF: 5 mg/ml; DMSO: 5 mg/ml | [form ]
powder
| [pka]
9.34±0.20(Predicted) | [color ]
red-brown
| [Water Solubility ]
Soluble in water (partly), methanol (10 mg/ml), and acetone. | [Usage]
Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens | [λmax]
496 nm | [BRN ]
48395 | [Stability:]
Light and Moisture Sensitive | [InChIKey]
GZAJOEGTZDUSKS-UHFFFAOYSA-N | [CAS DataBase Reference]
3326-34-9(CAS DataBase Reference) | [EPA Substance Registry System]
3326-34-9(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
Xn | [Risk Statements ]
R22:Harmful if swallowed.
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
3
| [F ]
10 | [TSCA ]
Yes | [HS Code ]
32049090 |
| Hazard Information | Back Directory | [Chemical Properties]
Dark Red Solid | [Uses]
5-aminofluorescein is also used to prepare FITC Isomer I (CDX-F0011, CDX-F0020). It is a chemical used as molecular probe and important as fluorescent dye and for derivatives. Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens. | [Uses]
Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens | [Uses]
Glycosaminoglycuronans react with 5-aminofluorescein to yield fluorescent derivatives. 5-aminofluorescein is also used to prepare FITC Isomer I (F-011, F-020). | [Definition]
ChEBI: A primary amino compound that is fluorescein carrying an amino substituent at C-5. Building block/intermediate for the synthesis of the fluorescent dye flourescein; also used to produce N-(fluorescein-5-yl)maleamic acid. | [Preparation]
The preparation of 5-aminofluorescein is carried out either by first saponification of 5-nitrofluorescein acetates with alcohol alkali, followed by reduction of the obtained 5-nitrofluorescein in a solution of alcohol alkali with hydrazine hydrate on a nickel catalyst to obtain 5-aminofluorescein , Or by boiling in an aqueous solution of sodium sulfide. The restoration of the nitro group and the saponification of acetates in the second case occurs in one stage.
| [General Description]
Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins. |
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