| Identification | Back Directory | [Name]
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+% | [CAS]
207738-08-7 | [Synonyms]
TMB 2HC1.xH2O
TMB*2HCL*2H2O
TMB (hydrochloride hydrate)
3,3',5,5'-tetramethylbenzidine·2HCl·2H2O
3,3μ,5,5μ-Tetramethylbenzidine hydrate dihydrochloride
3,3',5,5'-TetraMethylbenzidine dihydrochloride hydrate 97%
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%
3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate
3,3',5,5'-tetraMethylbiphenyl-4,4'-diaMine dihydrochloride hydrate (1:2:)
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate,TMB dihydrochloride
3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate Vetec(TM) reagent grade, >=98% | [EINECS(EC#)]
688-511-0 | [Molecular Formula]
C16H20N2.2ClH | [MDL Number]
MFCD00150104 | [MOL File]
207738-08-7.mol | [Molecular Weight]
313.27 |
| Chemical Properties | Back Directory | [Appearance]
Off-white fluffy powder | [Melting point ]
>300°C | [storage temp. ]
2-8°C | [solubility ]
Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml). | [form ]
Powder | [Sensitive ]
Moisture & Light Sensitive | [InChIKey]
NYNRGZULARUZCC-UHFFFAOYSA-N | [CAS DataBase Reference]
207738-08-7 |
| Hazard Information | Back Directory | [Chemical Properties]
Off-white fluffy powder | [Description]
TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000).1 The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB.1,2 Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase. | [Uses]
3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine. | [General Description]
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes. | [storage]
Store at -20°C |
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