| Identification | More | [Name]
D-LUCIFERIN POTASSIUM SALT | [CAS]
115144-35-9 | [Synonyms]
4,5-DIHYDRO-2-(6-HYDROXY-2-BENZOTHIAZOLYL)-4-THIAZOLECARBOXYLIC ACID POTASSIUM SALT
BEETLE LUCIFERIN, POTASSIUM SALT
D-(-)-2-[6'-HYDROXY-2'-BENZOTHIAZOLYL]DELTA-THIAZOLINE-4-CARBOXYLIC ACID POTASSIUM SALT
D-LUCIFERIN FIREFLY, POTASSIUM SALT
D-LUCIFERIN, MONOPOTASSIUM SALT
D-LUCIFERIN POTASSIUM SALT
D-LUCIFERIN SYNTHETIC POTASSIUM SALT
D-Luciferenpotassiumsalt
(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylicacid,potassiumsalt
(D)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid, Firefly, potassium salt
D-Luciferin*K
D-LUCIFERINPOTASSIUMSALT,99% | [EINECS(EC#)]
1592732-453-0 | [Molecular Formula]
C11H7KN2O3S2 | [MDL Number]
MFCD00044928 | [Molecular Weight]
318.41 | [MOL File]
115144-35-9.mol |
| Chemical Properties | Back Directory | [storage temp. ]
Inert atmosphere,Store in freezer, under -20°C | [solubility ]
DMF:16.7(Max Conc. mg/mL);52.45(Max Conc. mM)
DMSO:7.5(Max Conc. mg/mL);23.55(Max Conc. mM)
Ethanol:0.25(Max Conc. mg/mL);0.79(Max Conc. mM)
PBS (pH 7.2):10.0(Max Conc. mg/mL);0.31(Max Conc. mM)
Water:32.0(Max Conc. mg/mL);100.5(Max Conc. mM) | [form ]
A crystalline solid | [color ]
Light yellow | [Water Solubility ]
water: soluble | [BRN ]
5416262 | [InChI]
InChI=1/C11H8N2O3S2.K.H/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;;/h1-3,7,14H,4H2,(H,15,16);;/t7-;;/s3 | [InChIKey]
UMBKGTQQGYPQBE-OGFXRTJISA-M | [SMILES]
C([C@H]1CSC(C2=NC3C=CC(O)=CC=3S2)=N1)(=O)O.[KH] |&1:1,r| | [CAS DataBase Reference]
115144-35-9(CAS DataBase Reference) |
| Hazard Information | Back Directory | [Description]
D-Luciferin potassium salt(115144-35-9) is a water soluble form of D-Luciferin. D-Luciferin is a common bioluminescent substrate used for imaging of firefly luciferase expression with peak chemiluminescent emission at 560 nm. The ATP dependant luciferin oxidation is catalyzed by the Luciferase enzyme encoded by luc gene.
| [Chemical Properties]
Yellow powder | [Uses]
D-Luciferin Potassium Salt is a class of light-emitting heterocyclic compounds found in organisms that cause bioluminescence, such as firefly. This compound creates light through the process of oxidative decarboxylation by luciferase while ATP is present. In cell culture, D-Luciferin Potassium Salt is used to achieve highly sensitive bioluminescence detection. Recently, Luciferin was used to measure the uptake of mRNA in preparation of the vaccine against COVID-19. | [Application]
D-Luciferin may be used as a substrate in luciferase-based (EC 1.13.12.7) bioluminescence imaging and cell-based high-throughput screening applications. ATP can be measured with a reagent made up of D-luciferin and luciferase from firefly. D-Luciferin may be used and studied as a partial agonist for G protein-coupled receptor-35 (GPR35). Used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring. | [in vivo]
Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection[3].
Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection.
Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H2O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter. |
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