WITHAFERIN A synthesis
- Product Name:WITHAFERIN A
- CAS Number:5119-48-2
- Molecular formula:C28H38O6
- Molecular Weight:470.6
1159096-16-8
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Yield:-
Reaction Conditions:
with carbon dioxide in dimethyl sulfoxide at 37;
Steps:
Conversion of 2,3-dihydrowithaferin A-3β-0-sulfate to withaferin A in cell culture media; Stock solutions of 2,3-dihydrowithaferin A-3β-O-sulfate in DMSO (50 μL) were diluted into cell culture medium (950 μL) to achieve the indicated starting concentration, mixed thoroughly and the solution incubated in a CO2 incubator at 37 0C. Aliquots (100 μL) were withdrawn at 4, 16, and 24 h and subjected to HPLC analysis for 2,3- dihydrowithaferin A-3β-O-sulfate (RRT = 18.5 min) and withaferin A (RRT = 23.5 min) on a Kromasil Ci8 RP column (250 x 4.6 mm, 5 mm) with gradient elution using 40-100% aqueous MeOH and using an ELSD detector. An external standard curve method was used to calculate the concentration of each compound in the sampled aliquots (Khajuria, et al., J. Sep. Sci. (2004) 27: 541-546, incorporated herein by reference).
References:
WO2010/30395,2010,A2 Location in patent:Page/Page column 70
