PHENYLSERINE synthesis
- Product Name:PHENYLSERINE
- CAS Number:50897-27-3
- Molecular formula:C9H11NO3
- Molecular Weight:181.19
Yield:-
Reaction Conditions:
with sodium sulfate;2-hydroxyethanethiol in tetrahydrofuran;aq. phosphate buffer at 50 - 100; pH=7.5;Enzymatic reaction;Temperature;
Steps:
Serine, threonine, and phenylserine synthesis
The initial velocity of serine, threonine, or phenylserine synthesiswas measured in a 500 μL reaction with the followingbuffer solution components: 5.54 U SHMT, 50 mM phosphatebuffer pH 7.5, 25 mM sodium sulfate, 50 mM glycine,2 mM THF (for serine synthesis only), 50 mM formaldehyde(for serine synthesis), 10 mM acetaldehyde (for threoninesynthesis), 1 M benzaldehyde (for phenylserine synthesis),100 mM β-mercaptoethanol, and 0.3 μM PLP. The mixturewas incubated at various temperatures (50-100 °C) for 5 minbefore the enzymatic reaction was terminated with the additionof 1% TCA.
References:
Ma’ruf, Ilma Fauziah;Restiawaty, Elvi;Syihab, Syifa Fakhomah;Honda, Kohsuke;Akhmaloka [Amino Acids,2023,vol. 55,# 1,p. 75 - 88]
882741-46-0
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