ChemicalBook CAS DataBase List BIOTIN-XX-NHS

BIOTIN-XX-NHS synthesis

6synthesis methods

Product Name:BIOTIN-XX-NHS
CAS Number:89889-52-1
Molecular formula:C26H41N5O7S
Molecular Weight:567.7

6066-82-6 Synthesis

6066-82-6
753 suppliers
$5.00/10g

72040-64-3 Synthesis

72040-64-3
146 suppliers
$25.00/100mg

89889-52-1
110 suppliers
$20.00/5MG

Yield:89889-52-1 71%

Reaction Conditions:

with N-[3-(N,N-dimethylamino)-propyl]-N'-ethyl-carbodiimide hydrochloride in N,N-dimethyl-formamide at 20; for 48 h;

Steps:

2.1 2.1 Biotin-OSu-activated: S2204 (other batches S2223)

The commercial product 6-Biotinylamino-hexanoic acid (Biotin-Ahx-OH, 101 mg, 0.28 mmol) was dissolved in 4 ml of DMF (light suspension). N-hydroxy succinimide (NHS, 36 mg, 0.31 mmol) and N-(3-Dimethylaminopropyl)-N' ethyl-carbodiimide (EDC x HCI, 59 mg, 0.31 mmol.) were added to reaction mixture and stirred at room temperature for 2 days following disappearance of the acid on TLC analysis. Then, the organic solvent was evaporated under high vacuum and the obtained residue was dissolved in CH₂CI₂ (20 ml) and rapidly washed with 0.1 N HCI solution to remove excess reagents. The combined organic phase were washed with brine (20 ml), dried over anhydrous Na₂S0₄, filtered and concentrated to give a white solid Biotin-OSu activated (91 mg, yield 71 %), used immediately without further purification.

References:

WO2018/188990,2018,A1 Location in patent:Page/Page column 38

74124-79-1 Synthesis