Comparison of Bicine buffer and Tris buffer

1、 Similarities

Bicine and Tris are both a type of Good 'buffer used to regulate the pH value of laboratory liquids. They are both white crystalline powder in appearance and have good water solubility. When used, they need to be prepared into a solution in a certain proportion, and can be stored for a long time in a sealed environment at room temperature. Both can be used as buffer for electrophoresis experiments.

 

2、 Differences

1. Slight differences in appearance: Although bicine and tris are both white crystalline powders, careful observation reveals that bicine's crystalline form is elongated and looks more like monosodium glutamate; The white crystals of tris are shorter and thicker, appearing more like white sugar particles.

 

2. Different pH values: Bicine has an acidic solution with a pH value of 4.0 to 5.5 in aqueous solution at room temperature. According to the buffering coefficient, its buffering range is between pH values of 7.6 to 9.0; The pH value of tris in aqueous solution at room temperature is 10.5-12, which is an alkaline solution with a buffer range of pH 7-9.

 

3. Different uses: bicine is mainly used in laboratory research and development process and chemical production process, and is a common buffer solution in low-temperature biochemical work, which can be used for serum Guanine enzyme determination, enzyme reaction buffer, etc; The use of tris is more extensive, in addition to biochemical experiments, it can also be used as pharmaceutical intermediates, cosmetic additives, and new coatings and other fields.

 

4. Other differences: Tris is a commonly used basic organic chemical reagent, often configured with hydrochloric acid, boric acid, acetic acid, etc. as biological buffer with different pH values and uses, while bicine does not have so many derivatives. Ultimately, bicine and tris belong to two different substances, so there are still many differences in their physical and chemical properties.

 

Desheng is a professional manufacturer specializing in the research and production of biological buffering agents, with advanced production equipment and professional production technology. The production process is stable, the product batch differences are small, and the product purity can reach over 99%. In addition to the aforementioned bicine and tris, there are also dozens of biological buffering agents such as CAPS, mops, taps, etc., with a complete range of options for you to choose from. If you need anything, please feel free to contact me or click on the official website to check!