In electrophoresis experiments, biological buffers are essential. It can maintain stability during the electrophoresis process. Choosing a suitable biological buffer can also optimize the electrophoresis results, making the experimental results more accurate and reliable. This article will provide a detailed introduction to why biological buffering agents are used and which ones are suitable for electrophoretic experiments.

 

Electrophoresis is a method of separating charged molecules or ions from electrodes. In the electrophoresis experiment, it is usually necessary to add the protein or nucleic acid sample to be detected to the electrophoresis gel, and then under the action of the electric field, the sample will move to the electrode and stay at different positions, so as to judge the size and charge of the molecule for analysis and research.

 

In this process, the role of biological buffer is to help maintain the pH value and Ionic strength of gel. The pH value and Ionic strength of gel are one of the important parameters of electrophoretic separation. They will affect the dielectric properties of gel and the separation effect of samples. If the pH value or Ionic strength of the gel is unstable, the electrophoresis results will be inaccurate. Therefore, the use of biological buffer can effectively maintain the pH stability of gel.

 

Different biological buffers have different pH values, Ionic strength, buffer capacity and other aspects, so we need to select appropriate biological buffers according to actual needs. There are several types of buffers commonly used in electrophoresis experiments.

 

1. Tris buffer

Tris buffer is a commonly used biological buffer in electrophoresis experiments. Its chemical name is Tris, the PH value range is 7.0-9.0, and the Ionic strength is moderate, which will not affect the separation effect of the sample.

 

2. TAE buffer

TAE buffer solution is an acid buffer solution, which consists of three components: Tris, Acetic acid and EDTA. The pH value of TAE buffer is 7.0-8.3, and the Ionic strength is low. It is suitable for electrophoresis experiments with DNA or RNA

 

3. TBE buffer

TBE buffer solution is composed of three components: Tris, Boric acid and EDTA. The pH value of TBE buffer is 8.0-9.0, and the Ionic strength is moderate, which is suitable for DNA or RNA electrophoresis experiments.

 

Desheng is a manufacturer of biological buffering agents. Currently, there are over thirty types of biological buffering agents produced, which can meet the experimental needs of different scenarios. The product quality is stable, the purity is high, and technical support can be provided. If you need to make a purchase, please feel free to call and inquire about ordering at any time.