Can Tris buffer still be used when adjusted to acidity?

 

Tris, as a commonly used buffer for biochemical experiments, has a pH range of 7.5-9. As an alkaline solution, it can provide stable ion concentration within the pH range and maintain the sample under suitable conditions for reaction. But sometimes in experiments, improper operation may occur, such as accidentally mixing it into an acidic solution. Can this still be used? Below, we will introduce this issue specifically to everyone.

 

1、 What happens when Tris is adjusted to acidic?

When Tris buffer is diluted into an acidic solution, glycine in it reacts with phosphate ions to form inorganic salt precipitates. This precipitation can cause changes in the ion concentration in Tris, thereby affecting its buffering ability. In addition, under acidic conditions, Tris buffer may also react with other compounds, resulting in changes in its structure and properties.

 

2、 Can Tris be used when adjusted to acidity?

In fact, this answer is not absolute, it mainly depends on the specific experimental needs and conditions. If the experiment needs to be conducted under acidic conditions, it is necessary to prepare or derive hydrochloric acid buffer solution for use. However, it should be noted that the adjusted pH value and buffering capacity of the solution may change, and its applicability and stability need to be rechecked.

 

3、 Requirements for selecting Tris buffer in the experiment

1. The pH range of Tris: Tris is usually suitable for solutions with pH values ranging from 7.5 to 9. If the experiment needs to be conducted under acidic conditions, other buffer solutions that meet the requirements can be selected, and it is not recommended to directly adjust them to acidic solutions for use.

 

2. Tris concentration: Its concentration can also affect performance. Generally speaking, the higher the concentration, the stronger its buffering ability. However, excessive concentration may lead to ion precipitation or other adverse reactions. Therefore, when selecting the concentration of Tris buffer, it is necessary to determine the appropriate concentration based on experimental conditions.

 

3. Stability of Tris buffer solution: During use, the stability of the product is greater than anything else. Before purchasing, it is usually necessary to strictly inspect the appearance, packaging, or sample testing of Tris, and choose high-quality and pure Tris for use.

 

Although Tris is powerful, it cannot be used blindly. It is necessary to follow the instructions properly to fully ensure the accuracy of the experimental results. As an advantageous supplier of biological buffering agents, Desheng has a complete supply of products and can provide sample testing, providing great convenience for customers. If you are interested, please click on the website to inquire and purchase!