How to use buffer solutions correctly in chromatographic analysis?

 

In chromatographic analysis, the participation of buffer solutions is essential because it is used to balance acidity and alkalinity. If not used properly, serious consequences can occur, such as increased column pressure and abnormal retention time. Based on this situation, the use of buffer solutions in chromatographic analysis is a matter of great concern. How should it be used correctly? Below is a detailed introduction for everyone.

 

1、 Use of buffer solution before operation

Before the analysis operation, it is necessary to first rinse with a mobile phase without buffer. Generally, the mobile phase used for flushing has the same proportion as the mobile phase used for analysis, with the only difference being whether buffer is used in the mobile phase used for flushing. If a buffer salt solution is used, it is soluble in water and difficult to dissolve in organic solvents. Therefore, it is necessary to rinse before analysis, otherwise the chromatographic column may be damaged.

 

2、 Post operation handling

After the operation is completed, if the chromatographic column is not used for a long time, it needs to be rinsed again with organic solvent. Buffer solution is used in this process, which can provide counter ions for ion balance, and balance certain ionic strength and acid-base property in the flow process.

 

3、 Precautions for using buffer solution

1. When selecting buffer solutions, hydrochloride should not be used as it has adverse factors during the analysis process, which can lead to abnormal results.

2. Due to the fact that the finished buffer solution is generally in powder form, it needs to be prepared into a solution during use. It should be prepared and used immediately. If left for too long, bacteria can easily grow, which is not conducive to analysis.

3. After the experiment is completed, organic solvents should not be directly used excessively, otherwise it may cause buffer salts to precipitate and cause chromatographic column blockage.

4. During the trial period, observe the pH range in a timely manner to avoid too large a range.

5. If the buffer is continuously used during the operation, it is necessary to observe whether there is any precipitation. If there is precipitation, it should be cleaned to avoid blockage.

6. Choose to use a buffer solution with high purity and reliable quality to avoid experimental difficulties caused by poor quality.

 

The correct use of buffer solution in chromatographic analysis can effectively avoid many unnecessary troubles and ensure the validity of experimental results. However, it is important to pay attention to the operation and the selection of buffer solution.

 

As a professional manufacturer of biological buffer solutions, Desheng has complete equipment and a complete research and development team, resulting in buffer products with a purity of over 99% and stable performance. It has satisfactory results in both chromatographic analysis and other biochemical experiments. If you are interested, please click on the website to inquire and purchase!