Can separation gel be used to extract DNA from blood vessels in clinical practice?

 

Due to the strong stability of serum separation gel and its low adsorption capacity for proteins in serum, centrifugation can smoothly separate serum and blood clots, effectively preventing material exchange between them, facilitating the acquisition of high-quality serum and making blood testing results more reliable. At present, the use of separation hoses in clinical practice mainly involves biochemical or immunological testing based on serum samples. However, there are also cases where DNA is extracted from whole blood, and the quality of its extraction can also affect the experimental results. Will blood vessels containing separation gel be used for extraction in clinical practice? What are the extraction steps? Let's take a look together.

 

Due to the excellent separation of blood using separation hoses, both serum and plasma can be fully displayed, which is definitely possible for medical staff to extract DNA from them. The main methods are as follows:

 

1. Use a vacuum blood collection vessel containing separation gel to collect fasting blood samples in a sterile state. Immediately after collection, slowly invert and mix at 180 degrees for about 5 times, and then let it stand for about 20 minutes. Centrifuge at a radius of 3500r/min at 8cm for about 10 minutes. After centrifugation, DNA extraction and testing can be performed.

 

2. Directly aspirate the serum, add an appropriate amount of DNA concentrate and mix well. Then centrifuge at a radius of 5cm and a speed of 12000 r/min for about 10 minutes, and discard the clear solution.

 

3. Add a certain amount of DNA extraction solution and mix thoroughly in a 100 degree metal bath, then let it stand for about 6 hours, centrifuge for 5 minutes, and take the supernatant for PCR reaction. Place the sampled reaction tube onto the PCR machine, pre denature at 92 ° C for 3 minutes, and then circulate.

 

After research, it was found that using separation gel tubes to extract DNA basically does not affect the experimental and detection results, and the presence of separation gel is more conducive to rapid separation of serum and DNA testing, improving work efficiency. This is also a method worth promoting and using.

 

However, although it is very suitable for DNA experiments, special attention should also be paid to the quality of the separation gel. If the separation gel is of poor quality, even if it has a large effect, it is nonsense. It is recommended to use reliable serum separation gel manufacturers. As a supplier of blood vessel additives, Desheng has been developing serum separation gel since its establishment, which is convincing in terms of experience and product quality, and satisfactory in use. If you are interested, please click on the website to inquire about the details!